MIR494 reduces renal cancer cell survival coinciding with increased lipid droplets and mitochondrial changes

BMC Cancer
Punashi DuttaM Nanjundan

Abstract

miRNAs can regulate cellular survival in various cancer cell types. Recent evidence implicates the formation of lipid droplets as a hallmark event during apoptotic cell death response. It is presently unknown whether MIR494, located at 14q32 which is deleted in renal cancers, reduces cell survival in renal cancer cells and if this process is accompanied by changes in the number of lipid droplets. 769-P renal carcinoma cells were utilized for this study. Control or MIR494 mimic was expressed in these cells following which cell viability (via crystal violet) and apoptotic cell numbers (via Annexin V/PI staining) were assessed. By western blotting, MIR494 cellular responses were validated using MIR494 antagomir and Argonaute 2 siRNA. Transmission electron microscopy (TEM) was performed in MIR494-transfected 769-P cells to identify ultrastructural changes. LipidTOX green neutral lipid staining and cholesterol measurements were conducted to assess accumulation of lipids droplets and total cholesterol levels, respectively, in MIR494 expressing 769-P cells. Indirect immunofluorescence and western analyses were also performed to examine changes in mitochondria organization. Co-transfection of MIR494 mimic with siRNA targeting LC3B and ...Continue Reading

References

Mar 5, 2004·Cancer Research·Jinsong LiuRobert C Bast
Nov 9, 2004·Current Opinion in Cell Biology·Aimee L Edinger, Craig B Thompson
Jun 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lin ZhangGeorge Coukos
Jun 19, 2008·Genes & Development·Der-Fen SuenRichard J Youle
Jul 10, 2008·The Journal of Biological Chemistry·Joel M Goodman
Mar 13, 2009·The Journal of Biological Chemistry·Ruben K DagdaCharleen T Chu
Mar 17, 2009·Biochemical and Biophysical Research Communications·Masahiro ShibataYasuo Uchiyama
Jun 24, 2009·The Journal of Biological Chemistry·A Kathrin LutzKonstanze F Winklhofer
Sep 19, 2009·Nature Reviews. Genetics·Carlo M Croce
Oct 8, 2009·Human Molecular Genetics·Hsiuchen Chen, David C Chan
Apr 20, 2010·Advances in Cancer Research·Harry A Drabkin, Robert M Gemmill
Dec 24, 2010·Current Opinion in Lipidology·Carlos Fernández-HernandoKathryn J Moore
May 10, 2011·Annual Review of Nutrition·Kathryn J MooreCarlos Fernández-Hernando
Nov 2, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Won Kyu KimHoguen Kim
Nov 5, 2011·Nucleic Acids Research·Vera HuangLong-Cheng Li
Mar 31, 2012·Cell Death and Differentiation·J Boren, K M Brindle
Apr 11, 2012·Current Opinion in Lipidology·Jennifer Sacco, Khosrow Adeli
Apr 27, 2012·International Journal of Cell Biology·Rajat Singh, Ana Maria Cuervo
May 23, 2012·Metabolism: Clinical and Experimental·Elena FlowersBradley E Aouizerat
Jul 13, 2012·Cell Cycle·Sumitaka YamanakaFlorin M Selaru
Jul 25, 2012·Cellular and Molecular Life Sciences : CMLS·Leonidas BenetatosEvangelos Briasoulis
Sep 11, 2012·Autophagy·Steven K BackuesDaniel J Klionsky
Nov 13, 2012·Nature Cell Biology·Derrick GibbingsOlivier Voinnet
Mar 19, 2013·Journal of Lipid Research·Kasey C VickersAlan T Remaley
Nov 22, 2013·Journal of Atherosclerosis and Thrombosis·Takahiro HorieKoh Ono
Nov 23, 2013·PloS One·Ashutosh DharapRaghu Vemuganti
Mar 13, 2014·Journal of Gastroenterology and Hepatology·Weiling HeZunfu Ke
May 16, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marika ComegnaRaffaella Faraonio
Jun 15, 2014·FEBS Letters·Meiyan Jin, Daniel J Klionsky
Aug 6, 2014·International Journal of Molecular Sciences·Elisabeth Smolle, Johannes Haybaeck
Dec 17, 2014·Oral Oncology·Tatiana N Libório-KimuraEdward K L Chan
Feb 24, 2015·Current Opinion in Cell Biology·Akinori Eiyama, Koji Okamoto
Apr 11, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Juan WangHongyu Zhang

❮ Previous
Next ❯

Citations

Apr 1, 2018·Experimental Dermatology·Carina NicuRalf Paus

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
scraping
PCR
flow
Flow Cytometry
transmission electron microscopy
Assay
light

Software Mentioned

Image J
Graphpad Prism
Volocity 3D Imaging

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis