MircoRNA-1275 promotes proliferation, invasion and migration of glioma cells via SERPINE1.

Journal of Cellular and Molecular Medicine
Dong-Mei WuYuan-Lin Zheng

Abstract

This study was designed to explore the relationship between miR-1275 and SERPINE1 and its effects on glioma cell proliferation, migration, invasion and apoptosis. Differentially expressed miRNAs and mRNAs in glioma tissues were screened out by bioinformatic analysis. Dual-luciferase reporter gene assay was used to validate the targeted relationship between miR-1275 and SERPINE1. qRT-PCR was used to detect the expression of miR-1275 and SERPINE1 in glioma tissues. The expressions of SERPINE1 and p53 pathway-related proteins in glioma cells were detected by western blot. Glioma cell proliferation, apoptosis, migration and invasion were respectively detected by CCK-8 assay, flow cytometry, wound healing assay and transwell assay. Tumour xenograft model was developed to study the influence of miR-1275 and SERPINE1 on glioma growth in vivo. The results of microarray analysis, qRT-PCR and western blot showed that miR-1275 was low-expressed while SERPINE1 was high-expressed in glioma. Dual-luciferase assay showed that miR-1275 could bind to SERPINE1. Overexpression of miR-1275 could promote the p53 pathway-related proteins' expression. Highly expressed miR-1275 could repress the migration, proliferation and invasion of glioma cells wh...Continue Reading

References

Oct 24, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Lyndsay HarrisUNKNOWN American Society of Clinical Oncology
Aug 24, 2011·Endocrine-related Cancer·Deniz M ÖzataWeng-Onn Lui
Dec 27, 2011·Journal of Cancer Research and Clinical Oncology·Gianluigi MazzoccoliAda Piepoli
Dec 29, 2011·Frontiers in Bioscience (Landmark Edition)·Ying ZhangRoger Abounader
Jun 21, 2012·Genes & Development·William A Freed-Pastor, Carol Prives
Jun 22, 2012·Experimental Dermatology·R Matthew KleinPaul J Higgins
Aug 16, 2012·Genes & Cancer·Michael KarsyFred Moy
Apr 30, 2013·Molecular Neurobiology·Amanda Tivnan, Kerrie Leanne McDonald
Oct 2, 2013·Annual Review of Pathology·Gianpiero Di LevaCarlo M Croce
Oct 15, 2013·Cell·Cameron W BrennanUNKNOWN TCGA Research Network
Feb 18, 2014·Genes & Development·Nobuhiro OkadaLin He
Dec 9, 2014·Cancer Biology & Therapy·Sandra L Romero-CordobaAlfredo Hidalgo-Miranda
Mar 13, 2015·Molecular Carcinogenesis·Weiguang ZhangShiguang Zhao
Jan 27, 2016·Diabetes Research and Clinical Practice·Yiqiong MaGuohua Ding
Mar 26, 2016·Molecular Neurobiology·Dongsheng ZhaoZhou Fei
May 8, 2016·Journal of Molecular Endocrinology·Lingxia PangXirong Guo
Nov 30, 2016·Oncology Letters·Anna Agnieszka Klimczak-BitnerJanusz Szemraj
Dec 6, 2016·Biochemistry & Physiology·Ali S ArbabB R Achyut
Dec 21, 2016·EMBO Reports·Young Dong YooYong Tae Kwon
Apr 27, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yuan HuZiwei Du

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Citations

Apr 16, 2019·Frontiers in Microbiology·Affan A SherKevin M Coombs
Oct 31, 2020·Frontiers in Oncology·Ya-Ling LiBing Song
Jan 30, 2021·Journal of Experimental & Clinical Cancer Research : CR·Youqin XuWenhua Huang
Nov 7, 2020·Cancer Management and Research·Yunpeng XieWeixing Wang
Apr 4, 2021·Genes·Alaa SiddigWan Faiziah Wan Abdul Rahman
Jun 14, 2021·Cellular & Molecular Biology Letters·Guoying ZhangYiming Zeng

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Methods Mentioned

BETA
transfection
PCR
Assay
scraping
xenograft
antisense oligonucleotide

Software Mentioned

Graphpad Prism
Gene Set Enrichment Analysis ( GSEA )
GSEA
Limma
TargetScan
Bioconductor R
GraphPad

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