MiR&moRe2: A Bioinformatics Tool to Characterize microRNAs and microRNA-Offset RNAs from Small RNA-Seq Data

International Journal of Molecular Sciences
Enrico GaffoStefania Bortoluzzi

Abstract

MicroRNA-offset RNAs (moRNAs) are microRNA-like small RNAs generated by microRNA precursors. To date, little is known about moRNAs and bioinformatics tools to inspect their expression are still missing. We developed miR&moRe2, the first bioinformatics method to consistently characterize microRNAs, moRNAs, and their isoforms from small RNA sequencing data. To illustrate miR&moRe2 discovery power, we applied it to several published datasets. MoRNAs identified by miR&moRe2 were in agreement with previous research findings. Moreover, we observed that moRNAs and new microRNAs predicted by miR&moRe2 were downregulated upon the silencing of the microRNA-biogenesis pathway. Further, in a sizeable dataset of human blood cell populations, tens of novel miRNAs and moRNAs were discovered, some of them with significantly varied expression levels among the cell types. Results demonstrate that miR&moRe2 is a valid tool for a comprehensive study of small RNAs generated from microRNA precursors and could help to investigate their biogenesis and function.

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Citations

Dec 15, 2020·Briefings in Bioinformatics·Georges Pierre SchmartzAndreas Keller

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Methods Mentioned

BETA
sRNA-seq
RNA-seq
Illumina sequencing

Software Mentioned

ViennaRNA
Rstudio Server
Python3
DESeq2
bedtools
Python
pheatmap
MiRDeep2
LTS
ggplot2

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