miRNA-106a and prostate cancer radioresistance: a novel role for LITAF in ATM regulation

Molecular Oncology
Christianne HoeyStanley K Liu

Abstract

Recurrence of high-grade prostate cancer after radiotherapy is a significant clinical problem, resulting in increased morbidity and reduced patient survival. The molecular mechanisms of radiation resistance are being elucidated through the study of microRNA (miR) that negatively regulate gene expression. We performed bioinformatics analyses of The Cancer Genome Atlas (TCGA) dataset to evaluate the association between miR-106a and its putative target lipopolysaccharide-induced TNF-α factor (LITAF) in prostate cancer. We characterized the function of miR-106a through in vitro and in vivo experiments and employed transcriptomic analysis, western blotting, and 3'UTR luciferase assays to establish LITAF as a bona fide target of miR-106a. Using our well-characterized radiation-resistant cell lines, we identified that miR-106a was overexpressed in radiation-resistant cells compared to parental cells. In the TCGA, miR-106a was significantly elevated in high-grade human prostate tumors relative to intermediate- and low-grade specimens. An inverse correlation was seen with its target, LITAF. Furthermore, high miR-106a and low LITAF expression predict for biochemical recurrence at 5 years after radical prostatectomy. miR-106a overexpressi...Continue Reading

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Citations

Feb 19, 2020·Biogerontology·Maryam MajidiniaBahman Yousefi
May 18, 2019·Cellular and Molecular Neurobiology·Changlin HuangGuanghui Li
Dec 15, 2020·Critical Reviews in Oncology/hematology·Chiman MohammadiRezvan Najafi
Oct 19, 2019·Trends in Genetics : TIG·Junjie T HuaHousheng H He
Feb 4, 2021·Environmental Science and Pollution Research International·Yonglin ChenLan Yi
Feb 26, 2021·Frontiers in Pharmacology·Lan-Ya LiYan Cheng
Oct 15, 2020·Andrology·Maria Yu KonoshenkoPavel P Laktionov
May 1, 2021·Journal of Personalized Medicine·Katalin BalázsKatalin Lumniczky
Aug 21, 2021·Frontiers in Oncology·Sílvia SoaresRúben Fernandes

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Methods Mentioned

BETA
PCR
transfection
Assay
xenografts

Software Mentioned

bpg
PITA
TargetScan silico algorithm
miRDB
miRNAMap
miRMap
imagej
miRanda
RNAhybrid
miRwalk

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