MiRNA-126 expression inhibits IL-23R mediated TNF-α or IFN-γ production in fibroblast-like synoviocytes in a mice model of collagen-induced rheumatoid arthritis

Apoptosis : an International Journal on Programmed Cell Death
Jie GaoDongbao Zhao

Abstract

Both miR-126 and IL-23R affect rheumatoid arthritis (RA) procession. This study aimed to investigate the association of miR-126 and IL-23R and the possible modulation of miR-126 to RA pathogenesis. Serum, synovial tissue and synovial fluid were collected from patients with RA, and expression of miR-126, IL-23R, TNF-α and IFN-γ were detected. Fibroblast-like synoviocytes (FLS) was established using a collagen-induced arthritis mice model. The expression of miR-126 was manual intervened using pro-miR-126 and anti-miR-126 encoding lentivirus plasmids, or miR-126 agonists and corresponding negative controls. MiR-126 expression was inhibited in RA patients when compared with controls (P < 0.05). TNF-α and IFN-γ production and IL-23R expression were significantly upregulated in RA patients when compared to controls (P < 0.05). In pro-miR-126 treated FLS cells, the administration of pro-miR-126 plasmids upregulated miR-126, but inhibited IL-23R, TNF-α and IFN-γ expression or production. Moreover, the miR-126 agonist reversed the effects of the anti-miR-126 plasmid on FLS. These results revealed that miR-126 negative regulated the expression of IL-23R, TNF-α and IFN-γ. These results suggest the key impact of miR-126 on RA procession. M...Continue Reading

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Citations

Mar 20, 2020·International Journal of Molecular Sciences·Chary Lopez-PedreraCarlos Pérez-Sánchez
Oct 2, 2020·International Journal of Molecular Sciences·Tomasz KmiołekAgnieszka Paradowska-Gorycka
Jan 14, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Xiaojun DongDou Ding
May 18, 2021·Journal of Molecular Histology·Wei HuangAnying Li
Jul 19, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·YueJiao WangNing Zhang
Aug 12, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mohammad TaheriSoudeh Ghafouri-Fard

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Methods Mentioned

BETA
ELISA
FCS
PCR
protein assay

Software Mentioned

SPSS

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