miRNA-141 is a novel regulator of BMP-2-mediated calcification in aortic stenosis

The Journal of Thoracic and Cardiovascular Surgery
Bobby YanagawaSubodh Verma

Abstract

Bone morphogenetic protein-2 (BMP-2) is a major regulator of aortic valve calcification. MicroRNAs (miRNAs) are essential post-transcriptional modulators of gene expression and miRNA-141 is a known repressor of BMP-2-mediated osteogenesis. We hypothesized that miRNA-141 is a key regulator of aortic valve calcification. Porcine valvular interstitial cells were isolated, transfected with miRNA-141 or control, and stimulated with transforming growth factor-β. The BMP-2, extracellular signal-regulated kinase 1/2, and runt-related transcription factor 2 levels were determined by immunoblotting and reverse transcriptase polymerase chain reaction. To determine the role of miRNA-141 in bicuspid aortic valve disease, human bicuspid (n = 19) and tricuspid (n = 17) aortic valve leaflets obtained intraoperatively were submitted for GenoExplorer human microRNA array, immunoblotting, and histologic and immunohistochemical analyses. Stimulation of porcine aortic valvular interstitial cells with transforming growth factor-β induced morphologic alterations consistent with myofibroblastic transformation, BMP-2 signaling, and calcification. Transfection with miRNA-141 restored transforming growth factor-β-induced valvular interstitial cell activa...Continue Reading

References

Oct 15, 1995·Circulation·K D O'BrienC M Otto
Mar 21, 2001·Circulation·E R MohlerF S Kaplan
Aug 21, 2002·Circulation·Paul W M FedakJagdish Butany
Jul 19, 2005·Nature·Vidu GargDeepak Srivastava
Jul 20, 2007·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Cristina FayetAvrum I Gotlieb
Jan 17, 2008·Nature Reviews. Genetics·Witold FilipowiczNahum Sonenberg
Aug 22, 2009·Journal of Molecular and Cellular Cardiology·Vishal Nigam, Deepak Srivastava
Jul 14, 2010·Biochimica Et Biophysica Acta·Fazli WahidYou Young Kim
Apr 13, 2011·The Journal of Thoracic and Cardiovascular Surgery·Pedro R MorenoDavid H Adams

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Citations

Jun 1, 2013·Current Opinion in Nephrology and Hypertension·Ziad A Massy, Tilman B Drüeke
Jan 22, 2014·Nature Reviews. Cardiology·Joshua D HutchesonW David Merryman
Aug 9, 2016·The Journal of Pathology. Clinical Research·Patrick MathieuFrederick J Schoen
Aug 24, 2013·The Journal of Thoracic and Cardiovascular Surgery·Mi ZhangZhiyun Xu
Dec 10, 2015·Antioxidants & Redox Signaling·Joan Fernández EsmeratsHanjoong Jo
Jan 19, 2016·Global Cardiology Science & Practice·Alkistis KapelouzouDennis V Cokkinos
Mar 15, 2016·Progress in Biophysics and Molecular Biology·Xiaohong Liu, Zhiyun Xu
Aug 3, 2014·The Canadian Journal of Cardiology·Patrick Mathieu, Marie-Chloé Boulanger
Dec 3, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Soumen JanaAmir Lerman
May 24, 2016·International Journal of Molecular Sciences·Yei-Tsung ChenLieng-Hsi Ling
Sep 1, 2016·BioMed Research International·Jing ShiXiangqing Kong
Nov 4, 2016·Biochemical Genetics·Iolanda MunteanTheodora Benedek
Nov 24, 2016·Scientific Reports·Sean CoffeyGregory T Jones
Jul 16, 2016·International Journal of Molecular Sciences·Cécile OuryPatrizio Lancellotti
Apr 20, 2018·Journal of Cardiovascular Development and Disease·Froso SophocleousGiovanni Biglino
Feb 14, 2018·Journal of Cardiovascular Pharmacology·Wen-Juan NiXiao-Min Leng
Oct 7, 2017·Congenital Heart Disease·Andrew D Spearman
Jun 19, 2018·Journal of Developmental Origins of Health and Disease·B SiddeekU Simeoni
Sep 16, 2017·Journal of the American Heart Association·Arkady RutkovskiyJarle Vaage
May 20, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Chia-Ter ChaoHuei-Wen Chen
Sep 6, 2014·Arteriosclerosis, Thrombosis, and Vascular Biology·Katherine E YutzeyElena Aikawa
Oct 18, 2012·Circulation. Cardiovascular Genetics·Ratnasari PadangChristopher Semsarian
Jan 24, 2019·Archives of Pharmacal Research·Young-Kook Kim, Hyun Kook
Jul 28, 2017·Current Medicinal Chemistry·Konstantinos ToutouzasDimitris Tousoulis
May 30, 2019·Journal of Cellular and Molecular Medicine·Wenjie JiaoRongjian Xu
May 27, 2014·Expert Review of Cardiovascular Therapy·Patrick MathieuRihab Bouchareb
Jan 30, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Vishal PatelVishal Nigam
May 8, 2018·Journal of Cardiovascular Pharmacology·Wen-Juan NiXiao-Min Leng
Nov 22, 2018·Frontiers in Cardiovascular Medicine·Vinal Menon, Joy Lincoln
Mar 13, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Philip Roger GoodyFelix Jansen
Feb 27, 2017·Cardiovascular Engineering and Technology·Jack M HeathHanjoong Jo
Apr 13, 2017·Journal of Translational Medicine·Neus Martínez-MicaeloJosep M Alegret
Jan 7, 2017·Clinical Science·Casper F T van der VenElena Aikawa
May 8, 2020·International Journal of Molecular Sciences·Duk-Hwa KwonHyun Kook
Apr 14, 2017·Physiology·Yoginee SritharenJordan D Miller
Oct 11, 2020·Biomolecules·Gloria GaroffoloMaurizio Pesce

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