miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy

Cancers
Prashanth K B NageshMurali M Yallapu

Abstract

The therapeutic application of microRNA(s) in the field of cancer has generated significant attention in research. Previous studies have shown that miR-205 negatively regulates prostate cancer cell proliferation, metastasis, and drug resistance. However, the delivery of miR-205 is an unmet clinical need. Thus, the development of a viable nanoparticle platform to deliver miR-205 is highly sought. A novel magnetic nanoparticle (MNP)-based nanoplatform composed of an iron oxide core with poly(ethyleneimine)-poly(ethylene glycol) layer(s) was developed. An optimized nanoplatform composition was confirmed by examining the binding profiles of MNPs with miR-205 using agarose gel and fluorescence methods. The novel formulation was applied to prostate cancer cells for evaluating cellular uptake, miR-205 delivery, and anticancer, antimetastasis, and chemosensitization potentials against docetaxel treatment. The improved uptake and efficacy of formulations were studied with confocal imaging, flow cytometry, proliferation, clonogenicity, Western blot, q-RT-PCR, and chemosensitization assays. Our findings demonstrated that the miR-205 nanoplatform induces significant apoptosis and enhancing chemotherapeutic effects in prostate cancer cells....Continue Reading

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Citations

Jul 25, 2019·Journal of Cellular Biochemistry·Wenkai ZhouQichang Yan
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May 13, 2019·ACS Biomaterials Science & Engineering·Elham HatamiMurali Mohan Yallapu
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Methods Mentioned

BETA
transgenic
transfection
transfecting
circular dichroism
flow
confocal microscopy
flow cytometry
PCR
dynamic light scanning
transmission electron microscopy

Software Mentioned

GraphPad
Image analysis software
ImageJ
GraphPad Prism
ImageLabTM

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