miRNA-223-3p regulates NLRP3 to promote apoptosis and inhibit proliferation of hep3B cells

Experimental and Therapeutic Medicine
Lingfeng WanBoyu Xue

Abstract

Hepatocellular carcinoma (HCC) is a major malignant tumor type with a high incidence and mortality. Infection with hepatitis virus is a high-risk factor. Previous studies have demonstrated that microRNA (miR)-223 was downregulated in HCC tissues. NOD-like receptor family, pyrin domain containing 3 (NLRP3)-is a potential target of miR-223 and has a vital role in hepatitis infection. The present study was performed to investigate the role of miR-223 in the proliferation and apoptosis of HCC cells through regulating NLRP3. A dual luciferase reporter assay was performed to confirm the direct interaction between miR-223-3p and the 3' untranslated region of NLRP3 mRNA. Hep3B cells were then transfected with miR-223 mimics and the proliferation and apoptosis were determined by an MTT and a flow cytometric assay, respectively. The expression of NLRP3 and caspase-1 was analyzed at the mRNA as well as at the protein level by reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively. The secretion of interleukin (IL)-1β and IL-18 in the culture supernatants was measured by ELISA. The dual luciferase assay confirmed NLRP3 as a direct target of miR-223. Overexpression of miR-223 in hep3B cells sign...Continue Reading

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Citations

Apr 17, 2020·Bioscience Trends·Ganglei LiuLianwen Yuan
Oct 6, 2020·Journal of Immunology Research·Liliana R BalahuraMarieta Costache
May 24, 2019·Frontiers in Pharmacology·Gulcin TezcanSvetlana F Khaiboullina
Sep 18, 2021·Cancer Cell International·Liang WeiQinchuan Li

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Methods Mentioned

BETA
transfection
transfections
flow cytometry
Protein Assay
ELISA

Software Mentioned

SPSS
TargetScanHuman

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