miRNA-30e regulates abnormal differentiation of small intestinal epithelial cells in diabetic mice by downregulating Dll4 expression

Cell Proliferation
Ti-Dong ShanQi-Kui Chen

Abstract

Depression of the Notch/Hes1 pathway has been reported to play a role in abnormal differentiation of intestinal epithelial cells (IECs) in diabetes mellitus (DM). However, the mechanism by which this pathway influences IEC differentiation has remained unclear. In this study, we have investigated the role of microRNAs (miRNAs) in regulating the Notch/Hes1 pathway in IECs of DM mice. Integrated comparative miRNA microarray technology was used to determine the expression profile of miRNAs in IECs of DM mice. After bioinformatic analysis, an miRNA with altered expression levels, miRNA-30e, was identified as a candidate for regulating the Notch pathway in DM. A luciferase reporter assay confirmed that miRNA-30e targeted 3'-UTR of the Notch gene. The role of miRNA-30e in regulating Notch signalling was then explored by up- and downregulating its expression in vitro and in vivo. Abnormal differentiation of IECs in DM mice was associated with reduced activity of the Dll4/NICD/Hes1 signal pathway. Based on bioinformatic analyses, increased expression of miRNA-30e was identified as a potential candidate for regulating Notch signalling. miRNA-30e targeted the 3'-UTR of Dll4 and downregulated Dll4 expression in primary IECs and IEC-6 cells...Continue Reading

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Citations

Feb 18, 2018·Environmental Science and Pollution Research International·Ning XuZhange Yu
Jun 15, 2018·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Kathryn MirandaMitzi Nagarkatti
Jul 27, 2017·Molecular and Cellular Biochemistry·Ji-Hao XuQi-Kui Chen
Aug 4, 2020·Frontiers in Genetics·María Belén Ruiz-RosoAlberto Dávalos
Nov 11, 2020·Journal of Diabetes and Its Complications·Jie-Hao YuanJie-Yao Li

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