miRNA-378 reverses chemoresistance to cisplatin in lung adenocarcinoma cells by targeting secreted clusterin

Scientific Reports
Xuesong ChenLi Cai

Abstract

Cisplatin resistance is a major obstacle in the treatment of NSCLC, and its mechanism has not been fully elucidated. The objectives of the study were to determine the role of miR-378 in the sensitivity of lung adenocarcinoma cells to cisplatin (cDDP) and its working mechanism. With TargetScan and luciferase assay, miR-378 was found to directly target sCLU. miR-378 and sCLU were regulated in A549/cDDP and Anip973/cDDP cells to investigate the effect of miR-378 on the sensitivity and apoptotic effects of cDDP. The effect of miR-378 upregulation on tumor growth was analyzed in a nude mouse xenograft model. The correlation between miR-378 and chemoresistance was tested in patient samples. We found that upregulation of miR-378 in A549/cDDP and Anip973/cDDP cells significantly down-regulated sCLU expression, and sensitized these cells to cDDP. miR-378 overexpression inhibited tumor growth and sCLU expression in a xenograft animal model. Analysis of human lung adenocarcinoma tissues revealed that the cDDP sensitive group expressed higher levels of miR-378 and lower levels of sCLU. miR-378 and sCLU were negatively correlated. To conclude, we identified sCLU as a novel miR-378 target, and we showed that targeting sCLU via miR-378 may he...Continue Reading

References

Jan 29, 2003·The Journal of Biological Chemistry·Konstantin S LeskovDavid A Boothman
Jun 16, 2005·The Journal of Investigative Dermatology·Christoph HoellerBurkhard Jansen
Sep 15, 2007·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Jeffrey M AlbertBo Lu
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Daniel Y LeeBurton B Yang
May 29, 2009·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Nov 3, 2009·Advances in Cancer Research·Julie Y Djeu, Sheng Wei
Mar 31, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Amy J DavidoffMartin J Edelman
Feb 19, 2011·Journal of Experimental & Clinical Cancer Research : CR·Hai-Bo BianWei De
Jun 2, 2011·Cancer Chemotherapy and Pharmacology·Ching-Yuan ChengGwo-Tarng Sheu
Feb 18, 2012·Nature·Amaia Lujambio, Scott W Lowe
Jun 27, 2012·Expert Opinion on Biological Therapy·Liqin Du, Alexander Pertsemlidis
Aug 15, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Eric S KimZahid H Siddik
Feb 7, 2013·Cancer Chemotherapy and Pharmacology·Shailendra Kapoor
Jun 13, 2013·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Michela Garofalo, Carlo M Croce
Jul 10, 2013·Current Medicinal Chemistry·Subee TanJinbo Li
Aug 27, 2013·Lancet·Martin ReckKlaus F Rabe
Oct 2, 2013·Annual Review of Pathology·Gianpiero Di LevaCarlo M Croce

❮ Previous
Next ❯

Citations

Mar 10, 2017·Genes·Duncan Ayers, Jo Vandesompele
May 5, 2017·Anti-cancer Drugs·Marilina García-ArandaMaximino Redondo
Mar 1, 2018·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Ningning PengXu Guo
Feb 2, 2018·Nature Communications·Guermarie Velazquez-TorresMenashe Bar-Eli
Jul 21, 2017·Cellular & Molecular Biology Letters·Mingxi ZengChangming Kang
Sep 14, 2018·Frontiers in Oncology·Fabio CorràStefano Volinia
Nov 10, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Ying XiongChun Wang
Apr 9, 2020·Frontiers in Pharmacology·Jiabei ZhouLushan Yu
Jul 28, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jipeng LiWanjun Yu
Jan 2, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Prakash Priyadarshi PraharajSujit Kumar Bhutia
Jan 1, 2021·Cancers·János Tibor FeketeBalázs Győrffy

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
xenograft
antisense oligonucleotides
xenografts
fluorescence microscopy
RNA PCR
PCR

Software Mentioned

Bioinformatics
TargetScan
SPSS11

Related Concepts