miRNA-543 promotes cell migration and invasion by targeting SPOP in gastric cancer

OncoTargets and Therapy
Junfei XuXinguo Zhu

Abstract

Given the emerging role of microRNA (miRNA) in cancer progression, we investigated the role and mechanism of miRNA-543 (miR-543) in gastric cancer (GC). Real-time quantitative polymerase chain reaction was conducted to quantify the expression of miR-543. Luciferase reporter assay was used to confirm the association between speckle-type POZ protein (SPOP) and 3'-UTR. Moreover, the role of miR-543 and SPOP in GC was detected using transwell assays. In addition, we investigated the function of miR-543 in the epithelial-mesenchymal transition (EMT) progression. miR-543 was upregulated in GC. We identified SPOP as a direct target of miR-543, revealing its expression to be inversely correlated with miR-543 expression in GC tissues. Moreover, restoration of SPOP could inhibit miR-543-induced GC cell migration and invasion, whereas downregulation of miR-543 inhibited cell migration and invasion, which was partly abrogated by SPOP knockdown. Furthermore, our data also showed that miR-543 induced EMT of GC cells. Our results demonstrated that miR-543 functions as a crucial oncogenic miRNA in GC. It exerts strong tumor-promoting effects through targeting SPOP in GC cell migration and invasion.

Citations

Jul 19, 2019·Experimental and Therapeutic Medicine·Jie XuJianping Shao
Aug 30, 2019·Journal of Cellular Biochemistry·Lingmin ZhangLiping Song
Jun 17, 2020·Journal of Cellular Physiology·Cong ZhouShiwei Duan
Jul 3, 2020·Molecular and Cellular Biochemistry·Yin ZhangYuchun Pei
Jun 20, 2019·International Journal of Molecular Sciences·Irina V BureDmitry V Zaletaev
May 2, 2020·Nature Reviews. Urology·Zhiwei WangWenyi Wei
Jan 7, 2020·Molecular Cancer·Yizuo SongXueqiong Zhu
Dec 23, 2020·Technology in Cancer Research & Treatment·Feng LiangXin Lu
Nov 17, 2021·Archives of Gynecology and Obstetrics·Daoping ZhangMeirong Peng

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Methods Mentioned

BETA
transfection
ubiquitination

Software Mentioned

SPSS

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