miRNA-99b-3p functions as a potential tumor suppressor by targeting glycogen synthase kinase-3β in oral squamous cell carcinoma Tca-8113 cells

International Journal of Oncology
Kang HeChen Huang

Abstract

Dysregulation of microRNAs (miRNAs) has been associated with carcinogenesis in oral squamous cell carcinoma (OSCC). In the present study, we investigated the expression and function of miR-99b-3p in human OSCC. We found that the expression levels of miR-99b-3p decreased in 21 clinical OSCC samples (84%). Furthermore, ectopic expression of miR-99b-3p inhibited OSCC cell proliferation by downregulating glycogen synthase kinase-3β (GSK3β), an miR-99b-3p' target gene, at the mRNA and protein levels, both in vitro and in vivo. Moreover, the silencing of GSK3β recapitulated the cellular and molecular effects in a similar manner to the overexpression of miR-99b-3p, which included inhibition of OSCC cell proliferation and suppression of p65 (RelA) and G1 regulators (cyclin D1, CDK4 and CDK6) in vitro. Our data suggest that miR-99b-3p functions as a tumor suppressor in OSCC via GSK3β downregulation.

References

Oct 23, 2001·International Journal of Cancer. Journal International Du Cancer·D M ParkinP Pisani
Jan 16, 2002·Current Opinion in Genetics & Development·Alan Ho, Steven F Dowdy
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 23, 2004·Trends in Biochemical Sciences·Richard S Jope, Gail V W Johnson
Jun 2, 2004·Cellular and Molecular Life Sciences : CMLS·M A KuriakoseF A Chen
Sep 24, 2004·Cancer Genetics and Cytogenetics·Gokce A TorunerJames J Dermody
Nov 29, 2005·Science·Xantha Karp, Victor Ambros
Sep 5, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Andrei V OugolkovDaniel D Billadeau
Jan 9, 2007·Current Opinion in Genetics & Development·Paul Polakis
Feb 1, 2008·The New England Journal of Medicine·Carlo M Croce
Feb 8, 2008·BMC Genomics·Hui YeXiaofeng Zhou
Feb 13, 2008·Cell·Matthew S Hayden, Sankar Ghosh
Feb 24, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Kin Wai LaiUNKNOWN Singapore Gastric Cancer Consortium
Mar 30, 2010·Breast Cancer Research : BCR·Elizabeth O'Day, Ashish Lal
Jan 20, 2012·Nature Reviews. Cancer·Neil D Perkins
Feb 23, 2013·Thyroid : Official Journal of the American Thyroid Association·Matthias DettmerMarina N Nikiforova
Jun 5, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Emanuela GrassilliMarialuisa Lavitrano
Nov 12, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Le LuQiaoming Zhi
Sep 11, 2014·Molecular and Cellular Biochemistry·Chunxi LiuWuwei Gu

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Citations

Dec 18, 2018·Journal of Cellular Biochemistry·Jing LiQiuling Tong
Dec 17, 2017·Oncotarget·Yong-Jian WangYuan-Lin Zheng
Jan 5, 2018·Clinical Oral Investigations·Min CaoGary Guishan Xiao
May 28, 2020·International Journal of Molecular Sciences·Takashi TakeuchiSilvia Zappavigna
Nov 25, 2020·Wiley Interdisciplinary Reviews. RNA·Joseph Eniafe, Shuai Jiang
Oct 15, 2020·International Journal of Molecular Sciences·Iris Garrido-CanoPilar Eroles

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