Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres

Cell
Takeshi HayashiM Yanagida

Abstract

Centromeres contain specialized chromatin that includes the centromere-specific histone H3 variant, spCENP-A/Cnp1. Here we report identification of five fission yeast centromere proteins, Mis14-18. Mis14 is recruited to kinetochores independently of CENP-A, and, conversely, CENP-A does not require Mis14 to associate with centromeres. In contrast, Mis15, Mis16 (strong similarity with human RbAp48 and RbAp46), Mis17, and Mis18 are all part of the CENP-A recruitment pathway. Mis15 and Mis17 form an evolutionarily conserved complex that also includes Mis6. Mis16 and Mis18 form a complex and maintain the deacetylated state of histones specifically in the central core of centromeres. Mis16 and Mis18 are the most upstream factors in kinetochore assembly as they can associate with kinetochores in all kinetochore mutants except for mis18 and mis16, respectively. RNAi knockdown in human cells shows that Mis16 function is conserved as RbAp48 and RbAp46 are both required for localization of human CENP-A.

References

Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Apr 1, 1987·The Journal of Cell Biology·D K PalmerR L Margolis
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Oct 27, 1995·The Journal of Biological Chemistry·Y W Qian, E Y Lee
Feb 23, 1995·Nature·R HeimR Y Tsien
Oct 1, 1994·Molecular Biology of the Cell·K TakahashiM Yanagida
Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·M A Martínez-BalbásC Wu
Aug 3, 1999·European Journal of Biochemistry·T Krude
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·S HenikoffB van Steensel
Sep 21, 2002·Molecular and Cellular Biology·Quan-Wen JinUrsula Fleig
Jan 21, 2003·Genesis : the Journal of Genetics and Development·Takehito FuruyamaPeter J Harte
Feb 18, 2003·Molecular Biology of the Cell·Isabelle PotPhilip Hieter
Apr 30, 2003·The Journal of Cell Biology·Alison L PidouxRobin C Allshire
Jun 20, 2003·Nature·Steven Henikoff
Oct 21, 2003·Molecular Biology of the Cell·Vladimir S NekrasovJohn V Kilmartin
Oct 29, 2003·The Journal of Cell Biology·Stefan WestermannGeorjana Barnes
Nov 25, 2003·Genes & Development·Peter De WulfPeter K Sorger
Dec 6, 2003·The EMBO Journal·Maren ScharfenbergerJohannes Lechner

❮ Previous
Next ❯

Citations

Jul 14, 2011·Chromosoma·Marinela Perpelescu, Tatsuo Fukagawa
Jun 30, 2010·Molecular Genetics and Genomics : MGG·Gunjan D MehtaSantanu Kumar Ghosh
Jun 29, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Luis P ValenteLars E T Jansen
Jul 25, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Jan H BergmannWilliam C Earnshaw
Jul 24, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Ragini PhansalkarBarbara G Mellone
May 21, 2005·DNA Repair·Hiroshi Kimura
Mar 14, 2013·Trends in Cell Biology·Stefan Westermann, Alexander Schleiffer
Apr 9, 2005·Trends in Biochemical Sciences·Elaine DunleavyRobin Allshire
Apr 20, 2006·Nature Cell Biology·Daniel R FoltzDon W Cleveland
Jun 23, 2009·Nature Cell Biology·Christopher W CarrollAaron F Straight
Sep 8, 2009·Nature Cell Biology·Gianluca PegoraroTom Misteli
May 9, 2012·Nature Cell Biology·Alexander SchleifferStefan Westermann
Nov 13, 2008·Nature Reviews. Genetics·Robin C Allshire, Gary H Karpen
Dec 13, 2005·Nature Reviews. Molecular Cell Biology·Tomoyuki U TanakaKozo Tanaka
Dec 22, 2007·Nature Reviews. Molecular Cell Biology·Iain M Cheeseman, Arshad Desai
Apr 22, 2011·Nature Reviews. Molecular Cell Biology·Jolien S Verdaasdonk, Kerry Bloom
Nov 5, 2013·Nature Structural & Molecular Biology·Yuki OgiyamaKojiro Ishii
Dec 17, 2008·Molecular BioSystems·Jianhui Yao, Xiangwei He
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Muhammad ShuaibAli Hamiche
Nov 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Emilios K DimitriadisYamini Dalal
May 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Uhn-Soo Cho, Stephen C Harrison
Dec 26, 2012·Proceedings of the National Academy of Sciences of the United States of America·Marlyn GonzalezFei Li
May 3, 2011·The Journal of Biological Chemistry·Eun Shik ChoiRobin C Allshire
Oct 7, 2005·Molecular Biology of the Cell·Kimberly A CollinsSue Biggins
Oct 13, 2006·Molecular Biology of the Cell·Aki HayashiYasushi Hiraoka
Dec 14, 2007·Molecular Biology of the Cell·Yuko TakayamaKohta Takahashi
Jul 20, 2007·Molecular Biology of the Cell·Jonathan WongTony Hazbun
Jul 25, 2009·Molecular Biology of the Cell·Masahiro OkadaTatsuo Fukagawa
Aug 2, 2012·Briefings in Bioinformatics·Zexian LiuYu Xue
May 24, 2005·Nucleic Acids Research·Julian WalfridssonKarl Ekwall
May 18, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Kohta TakahashiShigeaki Saitoh
May 18, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mitsuhiro Yanagida
Apr 30, 2008·Genes & Development·Ji-Joon SongRobert E Kingston
Apr 16, 2008·Genome Research·Jun S SongXiangwei He
May 9, 2009·Genome Research·Danielle V IrvineRob Martienssen
Apr 7, 2011·Cold Spring Harbor Symposia on Quantitative Biology·B E BlackD W Cleveland

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.