Missense mutations define a restricted segment in the C-terminal domain of phytochrome A critical to its regulatory activity

The Plant Cell
Y XuP H Quail

Abstract

The phytochrome family of photoreceptors has dual molecular functions: photosensory, involving light signal perception, and regulatory, involving signal transfer to downstream transduction components. To define residues necessary specifically for the regulatory activity of phytochrome A (phyA), we undertook a genetic screen to identify Arabidopsis mutants producing wild-type levels of biologically defective but photochemically active and dimeric phyA molecules. Of eight such mutants identified, six contain missense mutations (including three in the same residue, glycine 727) clustered within a restricted segment in the C-terminal domain of the polypeptide. Quantitative photobiological analysis revealed retention of varying degrees of partial activity among the different alleles--a result consistent with the extent of conservation at the position mutated. Together with additional data, these results indicate that the photoreceptor subdomain identified here is critical to the regulatory activity of both phyA and phyB.

References

Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J R CherryR D Vierstra
Oct 1, 1994·Current Opinion in Genetics & Development·P H Quail
Dec 1, 1994·Plant Molecular Biology·T D Elich, J Chory
Jan 1, 1994·Annual Review of Genetics·A J MillarN H Chua
Jul 20, 1993·Journal of Molecular Biology·B Rost, C Sander
Sep 1, 1993·The Plant Cell·K DeheshP H Quail

❮ Previous
Next ❯

Citations

Jul 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P H Quail
Aug 1, 1996·Plant Molecular Biology·K Sakamoto, A Nagatani
Oct 6, 1997·The EMBO Journal·A C Mustilli, C Bowler
Jul 6, 2001·The Plant Journal : for Cell and Molecular Biology·J L WellerJ B Reid
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·D WagnerP H Quail
Nov 4, 1998·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P H Quail
Dec 1, 1996·Photochemistry and Photobiology·J M Staub, X W Deng
Feb 9, 2008·Annual Review of Plant Biology·Gabyong Bae, Giltsu Choi
Jan 1, 1997·Annual Review of Cell and Developmental Biology·C Fankhauser, J Chory
Dec 8, 2011·Bioscience, Biotechnology, and Biochemistry·Ah-Young ShinJeong-Il Kim
Jul 29, 2004·Genetics·Moira J SheehanThomas P Brutnell
Jun 28, 2000·Proceedings of the National Academy of Sciences of the United States of America·L Krall, J W Reed
Sep 8, 2010·Science China. Life Sciences·DaiYin Chao, HongXuan Lin
Jul 31, 2007·The Plant Journal : for Cell and Molecular Biology·Hidetoshi Uenaka, Akeo Kadota
Nov 10, 2011·Plant Signaling & Behavior·Stefan KircherEva Adam
May 17, 2018·Annual Review of Phytopathology·Gwyn A BeattieRegina S McGrane
Sep 27, 2018·The Plant Journal : for Cell and Molecular Biology·Hanna HelizonMathias Zeidler
Jun 27, 2003·Plant & Cell Physiology·Dae-Shik ChoMoon-Soo Soh
Sep 13, 2006·The Journal of Biological Chemistry·Julieta L MateosJorge J Casal
Dec 29, 1998·The Journal of Biological Chemistry·K EichenbergE Schäfer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.