Mistranslation can enhance fitness through purging of deleterious mutations

Nature Communications
Sinisa BratulicAndreas Wagner

Abstract

Phenotypic mutations are amino acid changes caused by mistranslation. How phenotypic mutations affect the adaptive evolution of new protein functions is unknown. Here we evolve the antibiotic resistance protein TEM-1 towards resistance on the antibiotic cefotaxime in an Escherichia coli strain with a high mistranslation rate. TEM-1 populations evolved in such strains endow host cells with a general growth advantage, not only on cefotaxime but also on several other antibiotics that ancestral TEM-1 had been unable to deactivate. High-throughput sequencing of TEM-1 populations shows that this advantage is associated with a lower incidence of weakly deleterious genotypic mutations. Our observations show that mistranslation is not just a source of noise that delays adaptive evolution. It could even facilitate adaptive evolution by exacerbating the effects of deleterious mutations and leading to their more efficient purging. The ubiquity of mistranslation and its effects render mistranslation an important factor in adaptive protein evolution.

References

Dec 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·C R Woese
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·V SiderakiH F Gilbert
Aug 28, 2003·Evolution; International Journal of Organic Evolution·Joanna Masel, Aviv Bergman
Jan 14, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jamie M BacherPaul R Schimmel
Jun 28, 2005·Nucleic Acids Research·Joost SchymkowitzLuis Serrano
Sep 7, 2005·Genetics·Reinhard BürgerMartin A Nowak
Apr 8, 2006·Science·Daniel M WeinreichDaniel L Hartl
Nov 14, 2006·Molecular Biology and Evolution·Michael G GiacomelliJoanna Masel
May 8, 2007·Journal of Molecular Biology·Nobuhiko TokurikiDan S Tawfik
May 9, 2008·PLoS Computational Biology·Nobuhiko TokurikiDan S Tawfik
May 16, 2008·Biology Direct·Dion J WhiteheadErich Bornberg-Bauer
Nov 22, 2008·Science·John EidStephen Turner
Sep 19, 2009·Nature Reviews. Genetics·D Allan Drummond, Claus O Wilke
Apr 24, 2010·FEMS Microbiology Reviews·Merijn L M SalverdaMiriam Barlow
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mor MeyerovichSigal Ben-Yehuda
Jan 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Etienne Rajon, Joanna Masel
Mar 17, 2011·PLoS Genetics·Merijn L M SalverdaJ Arjan G M de Visser
Apr 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Zhi Wang, Jianzhi Zhang
Apr 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Thomas E JonesTao Pan
May 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Li LiSusan A Martinis
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Li LiMichal T Boniecki
Oct 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jean-François GoutMichael Lynch
Jan 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Babak JavidEric J Rubin
Feb 26, 2014·Molecular Biology and Evolution·Elad FirnbergMarc Ostermeier
Jan 1, 2009·Language Learning and Development : the Official Journal of the Society for Language Development·Daniel J WeissAaron D Mitchel
Jun 27, 2014·The FEBS Journal·Gidi PelchovichUri Gophna
Sep 7, 2014·Genetics·Benjamin H Good, Michael M Desai

❮ Previous
Next ❯

Citations

Nov 15, 2017·Proceedings of the National Academy of Sciences of the United States of America·Merijn L M SalverdaJ Arjan G M de Visser
Aug 21, 2018·Annual Review of Cell and Developmental Biology·Roni RakYitzhak Pilpel
Jul 11, 2019·Open Biology·Anne WitzkyMichael Ibba
Jun 28, 2018·Nucleic Acids Research·Elizabeth B SawyerTeresa Cortes
Jul 15, 2018·Nucleic Acids Research·Stephanie M ZimmermanEric M Phizicky
Jan 26, 2021·Evolution; International Journal of Organic Evolution·Laasya SamhitaDeepa Agashe
Jun 20, 2021·Journal of Evolutionary Biology·Jia ZhengAndreas Wagner
Jul 14, 2021·Molecular Biology and Evolution·Jia ZhengAndreas Wagner
Oct 23, 2021·Biology Open·Neylen Del ToroGerardo Ferbeyre

❮ Previous
Next ❯

Datasets Mentioned

BETA
KT391064
KY727554
KY727555
KY741536

Methods Mentioned

BETA
PCR

Software Mentioned

ggplot2
SMRTAnalysis
ade4
BLASR
FoldX

Related Concepts

Related Feeds

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

© 2022 Meta ULC. All rights reserved