MITF-M regulates melanogenesis in mouse melanocytes

Journal of Dermatological Science
Tianzhi ChenChangsheng Dong

Abstract

Although the impact of the microphthalamia-associated transcription factor (Mitf) signaling pathway on melanocytes progression has been extensively studied, the specific molecular mechanisms behind MITF-M-enhanced melanin production in melanocytes still need to be clarified. In this study, we analyzed the levels of Mitf-M in skin tissues of different coat mice in order to further reveal the relationship between Mitf-M and skin pigmentation. To address the function of Mitf-M on melanogenesis, we have used an overexpression system and combined morphological and biochemical methods to investigate its localization in different coat color mice and pigmentation-related genes' expression in mouse melanocytes. The qRT-PCR assay and Western blotting analysis revealed that Mitf-M mRNA and protein were synthesized in all tested mice skin samples, with the highest expression level in brown skin, a moderate expression level in grey skin and the lowest expression level in black skin. Simultaneously, immunofluorescence staining revealed that MITF-M was mainly expressed in the hair follicle matrix and inner and outer root sheath in the skin tissues with different coat colors. Furthermore, overexpression of MITF-M led to increased melanin conte...Continue Reading

Citations

Sep 24, 2020·Biological Reviews of the Cambridge Philosophical Society·James D B O'SullivanRalf Paus
Aug 18, 2020·Neural Plasticity·Linjun ChenShiming Yang
Aug 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nurshafika Mohd SakehSyahida Ahmad
Jan 21, 2021·Animals : an Open Access Journal From MDPI·Xianbo JiaSongjia Lai
Apr 4, 2021·International Journal of Molecular Sciences·Urszula KazimierczakAndrzej Mackiewicz
Jul 3, 2021·International Journal of Molecular Sciences·Yekatsiaryna HushchaMonica De Mattei
Jun 29, 2021·Frontiers in Molecular Biosciences·Shuaishuai HuXinsheng Wu
Jul 25, 2021·International Journal of Molecular Sciences·Ilandarage Menu Neelaka MolagodaGi-Young Kim

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