May 10, 1977

Mithramycin and DIPI: a pair of fluorochromes specific for GC-and AT-rich DNA respectively

Human Genetics
W SchnedlG Stranzinger

Abstract

The AT specificity of the fluorochromes DIPI and DAPI and the GC specificity of mithramycin are evidenced by observations in human, mouse, and bovine chromosomes. DIPI and DAPI produce a pattern similar to Hoechst 33258 in all three species, whereas mithramycin results in a reverse pattern. The AT-rich centromeric heterochromatin in mouse is brilliantly stained by DIPI or DAPI and remains nearly invisible after mithramycin staining. In the GC-rich centromeric heterochromatin of cattle the opposite behavior is observed.

Mentioned in this Paper

Bos taurus
Plicamycin
Heterochromatin
4',6-bis(2'imidazolinyl-4',5'-H)-2-phenylindole
DAPI
Chromosomes
Staining and Labeling
Fluorochromes
NCKIPSD
Bos indicus

About this Paper

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