PMID: 8947076Nov 1, 1996Paper

Mitochondria act as a reservoir for the basic amine HIPDM in the lung

The European Respiratory Journal
M MiniatiM Pistolesi

Abstract

The lungs are a site for the uptake, accumulation, and storage of exogenous basic amines. The compound N-N-N'-trimethyl-N'-(2-hydroxy-3-methyl-5-iodobenzyl)-1,3 propanediamine (HIPDM) is a basic amine that can be labelled with radioactive iodine and detected by external counting. Intravenously injected 123I-HIPDM is extracted by the human lung, where it is retained in a slowly effluxable pool. In the present study, we measured HIPDM lung kinetics and subcellular distribution in rabbits given i.v. 125I-HIPDM. Rabbits were killed from 2 min to 5 h after injection, and the radioactivity retained in their lungs was measured. Subcellular lung fractions (nuclear, mitochondrial, lysosomal, microsomal and postmicrosomal supernatant) were assayed for HIPDM radioactivity, protein content, and distribution of specific marker enzymes. HIPDM lung clearance in rabbits was nearly identical to that of humans. Virtually all the HIPDM radioactivity in lungs (98+/-1%) was associated with subcellular membranous structures. The highest HIPDM specific radioactivity was found in the mitochondrial fraction, and the subcellular distribution profile closely resembled that of the mitochondrial marker enzyme succinate cytochrome c reductase. No redistribu...Continue Reading

Citations

Aug 21, 2007·Annals of Nuclear Medicine·Akihiro TakanoTetsuya Suhara
Jul 1, 1999·Clinical and Experimental Pharmacology & Physiology·R N Upton, D J Doolette
Jan 9, 2013·Expert Opinion on Drug Metabolism & Toxicology·Xin LiuMichael S Roberts
Feb 24, 2001·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·D ZhengA Martinez
Jun 15, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jianbo LiZhirong Zhang
Nov 24, 1999·Journal of Applied Physiology·S H AudiC A Dawson
Jun 16, 1999·Journal of Applied Physiology·D L RoerigC A Dawson
Jun 16, 2009·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Jocelyn DupuisAlain Fournier

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