Mitochondria are morphologically and functionally heterogeneous within cells

The EMBO Journal
Tony J CollinsM D Bootman

Abstract

We investigated whether mitochondria represent morphologically continuous and functionally homogenous entities within single intact cells. Physical continuity of mitochondria was determined by three-dimensional reconstruction of fluorescence from mitochondrially targeted DsRed1 or calcein. The mitochondria of HeLa, PAEC, COS-7, HUVEC, hepatocytes, cortical astrocytes and neuronal cells all displayed heterogeneous distributions and were of varying sizes. There was a denser aggregation of mitochondria in perinuclear positions than in the cell periphery, where individual isolated mitochondria could be seen clearly. Using fluorescence-recovery after photobleaching, we observed that DsRed1 and calcein were highly mobile within the matrix of individual mitochondria, and that mitochondria within a cell were not lumenally continuous. Mitochondria were not electrically coupled, since only individual mitochondria were observed to depolarize following irradiation of TMRE-loaded cells. Functional heterogeneity of mitochondria in single cells was observed with respect to membrane potential, sequestration of hormonally evoked cytosolic calcium signals and timing of permeability transition pore opening in response to tert-butyl hydroperoxide....Continue Reading

References

Jan 1, 1988·Annual Review of Cell Biology·L B Chen
Aug 5, 1974·Biochemical and Biophysical Research Communications·J T BrandtM L Vorbeck
Aug 11, 1995·Cell·G HajnóczkyA P Thomas
Oct 1, 1994·Journal of Bioenergetics and Biomembranes·K K Gunter, T E Gunter
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·G A RutterR M Denton
Feb 24, 1997·The Journal of Cell Biology·D F BabcockB Hille
May 5, 1997·The Journal of Cell Biology·M HothR S Lewis
Apr 17, 1998·Biophysical Journal·J HüserL A Blatter
Sep 24, 1998·Biophysical Journal·C FinkL M Loew
Dec 18, 1998·The Journal of Biological Chemistry·S RickenR Nitschke
Jan 6, 1999·Biophysical Journal·R C Scaduto, L W Grotyohann
Feb 20, 1999·The Journal of Biological Chemistry·K M HeiskanenA L Nieminen
Apr 27, 2000·Trends in Biochemical Sciences·G A Rutter, R Rizzuto
Dec 15, 2000·Cell Calcium·F De GiorgiF Ichas
Feb 13, 2001·Trends in Biochemical Sciences·V P Skulachev
May 3, 2001·The Journal of Biological Chemistry·T J CollinsM D Bootman

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Citations

Dec 25, 2004·Die Naturwissenschaften·Carlo Bertoni-FreddariMarta Balietti
Jun 2, 2005·Analytical and Bioanalytical Chemistry·Karen J OlsonEdgar A Arriaga
Jul 6, 2007·Pflügers Archiv : European journal of physiology·Wolfgang F GraierRoland Malli
Sep 2, 2011·Pflügers Archiv : European journal of physiology·Vera C KeilMichael Müller
Mar 3, 2012·Pflügers Archiv : European journal of physiology·Lukas N GroschnerWolfgang F Graier
Mar 8, 2006·Biomechanics and Modeling in Mechanobiology·M M KnightD L Bader
Oct 20, 2005·Apoptosis : an International Journal on Programmed Cell Death·M SaundersB E Sawaya
May 8, 2007·Bioscience Reports·Claudia NestiLuigi Murri
Jan 14, 2010·Journal of Bioenergetics and Biomembranes·Grigory I Belogrudov
Dec 1, 2005·Journal of Biological Physics·Sergey IvanchenkoG Ulrich Nienhaus
May 11, 2005·Toxicology and Applied Pharmacology·Gerhard KrumschnabelBettina Hofer
Feb 26, 2003·Cell Calcium·Gavriel DavidEllen F Barrett
Jul 31, 2003·Cell Calcium·Stefano L SensiKyle R Gee
Jan 18, 2003·Brain Research. Molecular Brain Research·Alberto Chiarugi
Feb 5, 2003·Current Opinion in Biotechnology·Kathryn M Fuller, Edgar A Arriaga
Aug 13, 2004·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Nhu-An PhamBrian H Robinson
Nov 26, 2010·Nature Reviews. Molecular Cell Biology·Benedikt Westermann
Jun 10, 2006·Cell Death and Differentiation·J-C Martinou, R J Youle
Dec 7, 2006·Reproduction, Fertility, and Development·Barry D Bavister
Jul 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Felix T KurzAntonis A Armoundas
Jul 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Christian A WurmStefan Jakobs
Nov 3, 2010·Molecular & Cellular Proteomics : MCP·Melanie Y WhiteJennifer E Van Eyk
May 30, 2012·The Journal of General Physiology·Jaime Santo-Domingo, Nicolas Demaurex
Aug 12, 2003·Journal of Neurotrauma·Jose L Perez VelazquezMarina V Frantseva
Apr 26, 2008·Antioxidants & Redox Signaling·Giovanni Benard, Rodrigue Rossignol
Feb 4, 2009·Antioxidants & Redox Signaling·David F Stowe, Amadou K S Camara
Jul 1, 2010·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Asen BagashevKathleen E Sullivan
May 19, 2006·Rejuvenation Research·Carlo Bertoni-FreddariMarta Balietti
May 6, 2009·Human Reproduction Update·João Ramalho-SantosAlexandra Amaral
Nov 6, 2008·Acta Physiologica·A SpätG Szanda
Jan 15, 2004·Science's STKE : Signal Transduction Knowledge Environment·Rosario RizzutoTullio Pozzan
Apr 3, 2004·Molecular and Cellular Biology·Nathan A Elliott, Michael R Volkert

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