PMID: 8603096Mar 27, 1996Paper

Mitochondria are the functional intracellular target for a photosensitizing boronated porphyrin

Biochimica Et Biophysica Acta
A D MundayP Nagley

Abstract

A photosensitizing boron-containing porphyrin derivative denoted BOPP, which is selectively localised into mitochondria, has been tested on Namalwa cells, in each of two genetic configurations: rho+ cells containing normal mtDNA and mitochondrial respiratory functions, or rho0 cells lacking mtDNA and devoid of mitochondrial oxidative phosphorylation. After short-term cellular uptake for 18 h, BOPP (30 micrograms/ml) was not cytotoxic, but did show marked phototoxicity in Namalwa rho+ cells, concomitant with substantial reduction of mitochondrial respiratory activity. After long-term (3 days or more) exposure to BOPP without light, growth of Namalwa rho+ cells was inhibited at concentrations significantly above 30 micrograms/ml. At such concentrations BOPP was shown to have direct inhibitory effects on mitochondrial azide-sensitive respiration of p+ cells. By contrast, BOPP showed neither cytotoxic nor phototoxic effects in rho0 cells. These results indicate functional mitochondria to be a major cellular target in vivo after BOPP uptake and photoactivation.

References

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Citations

May 30, 2001·Advanced Drug Delivery Reviews·J Morgan, A R Oseroff
Dec 1, 1996·Photochemistry and Photobiology·P G SpizzirriK P Ghiggino
Mar 8, 2011·Biochimica Et Biophysica Acta·Fernanda RicchelliPaolo Bernardi
Mar 22, 2006·Journal of Photochemistry and Photobiology. B, Biology·M EgyekiG Csík
Jul 12, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Hongyou ZhaoQun Chen
Feb 24, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M A RosenthalA H Kaye
Dec 26, 2001·Chemical Reviews·H Ali, J E van Lier
Feb 19, 2002·Chemical Reviews·Albert H. SolowayJ. Gerald Wilson

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