PMID: 8596689Feb 1, 1996Paper

Mitochondria contribute to Ca2+ removal in smooth muscle cells

Pflügers Archiv : European journal of physiology
R M Drummond, F S Fay

Abstract

Recent evidence, from a variety of cell types, suggests that mitochondria play an important role in shaping the change in intracellular calcium concentration ([Ca2+]i) that occurs during physiological stimulation. In the present study, using a range of inhibitors of mitochondrial Ca2+ uptake, we have examined the contribution of mitochondria to Ca2+ removal from the cytosol of smooth muscle cells following stimulation. In voltage-clamped single smooth muscle cells, we found that following a 8-s train depolarizing pulses, the rate of Ca2+ extrusion from the cytosol was reduced by more than 50% by inhibitors of cytochrome oxidase or exposure of cells to the protonophore carbonyl cyanide P-trifluoromethoxy-phenylhydrazone. Using the potential-sensitive indicator-tetramethyl rhodamine ethyl ester, we confirmed that the effect of these agents was associated with depolarization of the mitochondrial membrane. Since, the primary function of the mitochondria is to provide the cell's ATP, it could be argued that it is the ATP supply to the ion pumps which is limiting the rate of Ca2+ removal. However, experiments carried out with the mitochondrial Ca2+ uniporter inhibitor ruthenium red produced similar results, while the ATP synthetase i...Continue Reading

References

Jul 1, 1978·The Journal of General Physiology·F J BrinleyA Scarpa
Jan 1, 1991·Pharmacology & Therapeutics·L MissiaenR Casteels
Dec 1, 1991·The American Journal of Physiology·C M YangH J Chien
Dec 1, 1991·Journal of Bioenergetics and Biomembranes·R G Hansford
Jan 1, 1991·Physiological Reviews·E Carafoli
Jan 1, 1990·Annual Review of Neuroscience·P Hess
Jan 1, 1990·Annual Review of Physiology·R M Denton, J G McCormack
May 1, 1990·The American Journal of Physiology·T E Gunter, D R Pfeiffer
Sep 1, 1989·The Journal of Physiology·T J BiscoeM Valdeolmillos
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A V SomlyoA Scarpa
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S YagiF S Fay
Oct 1, 1987·The American Journal of Physiology·P L Becker, F S Fay
Feb 5, 1973·Biochemical and Biophysical Research Communications·C S RossiE Carafoli
Jan 1, 1982·Methods in Enzymology·F S FayP Merriam
Sep 1, 1994·The Journal of Cell Biology·R RizzutoT Pozzan
Aug 1, 1994·The American Journal of Physiology·T E GunterC E Gavin
Feb 1, 1994·Pflügers Archiv : European journal of physiology·J G McCarronF S Fay
Jan 28, 1993·Nature·M J Berridge
Jan 1, 1993·The Journal of Physiology·J W BassaniD M Bers

❮ Previous
Next ❯

Citations

Jan 23, 1999·BioFactors·P PintonR Rizzuto
May 5, 2012·Pflügers Archiv : European journal of physiology·John G McCarronSusan Chalmers
Apr 28, 2004·Cell Calcium·T B BoltonV Pucovsky
Mar 26, 2004·Biophysical Journal·Etienne Roux, Marko Marhl
Apr 20, 2000·European Journal of Pharmacology·F FusiG Sgaragli
Mar 17, 1999·European Journal of Pharmacology·G V Petkov, K K Boev
May 26, 1998·Brain Research. Brain Research Reviews·P B Simpson, J T Russell
Dec 14, 1999·Biochimica Et Biophysica Acta·T B ShererJ B Tuttle
Aug 26, 1998·Trends in Microbiology·M CouturierL Van Melderen
Dec 15, 2000·Cell Calcium·T E GunterK Gunter
Mar 24, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Sergey I DikalovA Maziar Zafari
May 23, 1998·The Journal of Physiology·D V GordienkoM B Cannell
May 12, 1998·The Journal of Physiology·M J Taggart, S Wray
Jan 12, 2013·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·John G McCarronSusan Chalmers
Apr 28, 2004·Cell Calcium·Damon PoburkoCornelis van Breemen
Feb 8, 2006·Autonomic Neuroscience : Basic & Clinical·Dong-Yoon LimSoichi Miwa
Jan 18, 2006·Mechanisms of Ageing and Development·Cristiane del CorssoSean M Wilson
Aug 4, 1998·Current Opinion in Neurobiology·D F Babcock, B Hille
Nov 26, 1998·Experimental Cell Research·G V KulkarniC A McCulloch
Apr 1, 1999·Annual Review of Physiology·T B BoltonD V Gordienko
Sep 24, 1998·Biophysical Journal·T Kamishima, J G McCarron
Apr 14, 2017·Journal of Hypertension·Iago Méndez-LópezJ Fernando Padín
Jan 21, 2010·Physiological Reviews·Susan Wray, Theodor Burdyga
Mar 29, 2014·Pharmacological Reviews·Marie BillaudBrant E Isakson
Jan 11, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tania SzadoCornelis van Breemen
May 24, 2000·The Journal of Biological Chemistry·A Nassar, A W Simpson
May 3, 2001·The Journal of Biological Chemistry·T J CollinsM D Bootman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.