Mitochondria-Immobilized Near-Infrared Ratiometric Fluorescent pH Probe To Evaluate Cellular Mitophagy

Analytical Chemistry
Xiaoyi LiHuimin Ma

Abstract

Mitochondria, powerhouses of cells, possess a weakly alkaline environment. Various stress stimulations may lead to mitophagy, which further gives a rise to mitochondrial acidification and disfunction. Therefore, monitoring mitochondrial pH alterations is of great importance to better elucidate their role in the cellular metabolism. Toward this end, a number of mitochondrial fluorescent pH probes have been proposed, but most of them are based on electrostatic attraction and readily leak out from the mitochondria during mitophagy with decreased membrane potential, thus failing to accurately measure the pH changes. In this work, we report a mitochondria-immobilized ratiometric fluorescent pH probe, which allows the quantitative measurements of mitochondrial pH. The probe was designed and prepared by introducing a reactive benzyl chloride into a positively charged near-infrared hydroxyl-hemicyanine. The cationic property facilitates the probe to be quickly enriched into mitochondria, the hydroxyl group is responsible for producing a reversible ratiometric fluorescence signal, and benzyl chloride is used to react with nucleophiles for immobilizing the probe in mitochondria. Taking these advantages of the probe, the mitochondrial pH ...Continue Reading

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Citations

Sep 17, 2020·Chemical Communications : Chem Comm·Huawei NiuYufen Zhao
Sep 16, 2020·Analytical Methods : Advancing Methods and Applications·Lingling LiWen Zhang
Jan 15, 2021·Chemical Communications : Chem Comm·Shan WangKang-Kang Yu
Nov 20, 2019·Analytical Chemistry·Xiaoxue ZouShoufa Han
Feb 6, 2021·Topics in Current Chemistry·Yuexiang MaJie Zhang
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May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yunnan YanHaiying Liu
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Jun 28, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yu Zhu YangHong Qun Luo
Jul 2, 2020·Analytical Chemistry·Lu JiaQing-Zheng Yang
Sep 30, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jiang-Lin WangFeng-Lei Jiang
Oct 26, 2021·Chembiochem : a European Journal of Chemical Biology·Pankaj E HandeSantosh J Gharpure

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