PMID: 9435269Jan 22, 1998Paper

Mitochondria-rich cells and voltage-activated chloride current in toad skin epithelium: analysis with the scanning vibrating electrode technique

The Journal of Membrane Biology
W NagelA M Shipley

Abstract

The pathway for the voltage-activated chloride current across isolated toad skin was analyzed using a scanning 2D-vibrating voltage probe technique, which permits discrimination of local current peaks if their origins are more than 50 microns apart. The epithelium was separated from the corial connective tissue after enzymatic digestion with collagenase. Cl- current was activated by voltage clamping the transepithelial potential to 60-100 mV, serosa positive. Activated inward current was between 85 and 450 microA/cm2. In more than 25 tissue areas of 150 x 100 microns from 10 animals, which were automatically scanned with the vibrating probe, between 0 and 4 peaks of elevated local current (up to 800 microA/cm2) could be identified in individual fields. The density of current peaks, which were generally located at sites of mitochondria-rich (MR) cells, was less than 10% of the density of microscopically identified MR cells. The total current across individual sites of elevated conductance was 3.9 +/- 0.6 nA. Considering the density of peaks, they account for 17 +/- 2.5% of the applied transepithelial clamping current. The time course of current activation over previously identified conductive sites was in most cases unrelated to...Continue Reading

Citations

May 29, 2003·Journal of Insect Physiology·K W. BeyenbachM -J. Yu
Aug 12, 1999·Acta histochemica·G ZacconeU Katz
Apr 27, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·W Nagel, U Katz
Apr 26, 2000·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·U KatzS Gabbay
Jul 20, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Manuel Claro de Toledo, Sonia Malheiros Lopes Sanioto
Sep 8, 2001·American Journal of Physiology. Cell Physiology·W NagelU Katz
Nov 8, 2002·Biochimica Et Biophysica Acta·Niels J WillumsenE Hviid Larsen

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