Mitochondria sense with different kinetics the calcium entering into HeLa cells through calcium channels CALHM1 and mutated P86L-CALHM1

Biochemical and Biophysical Research Communications
Ana J Moreno-OrtegaMaría F Cano-Abad

Abstract

The novel Ca(2+) channel CALHM1 (Calcium Homeostasis Modulator 1) generates cytosolic Ca(2+) transients ([Ca(2+)](c)) that regulate the production of amyloid beta (Abeta). Its mutated channel P86L-CALHM1 has been associated to Alzheimer's disease (AD). Using cytosolic- and mitochondrial-targeted aequorins, we have investigated here whether mitochondria sense with similar or different kinetics the Ca(2+) entering into Hela cells and the Ca(2+) released from the endoplasmic reticulum (ER), in control and in cells transfected with CALHM1 and P86L-CALHM1. We have shown that mitochondria sense Ca(2+) entry in the three cell types; however, the [Ca(2+)](c) and mitochondrial Ca(2+) transients [Ca(2+)](m) had substantially slower kinetics in cells expressing P86L-CALHM1. Mitochondria also sensed the ER Ca(2+) released by histamine, but in CALHM1 and P86L-CALHM1 cells the kinetics was faster than that of control cells. Data are compatible with the idea that mutated CALHM1 may cause mitochondrial Ca(2+) overload, suggesting how these cells may become more vulnerable to apoptotic stimuli.

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Citations

Aug 4, 2012·ACS Chemical Neuroscience·Laura González-LafuenteCristóbal de Los Ríos
Nov 23, 2012·ACS Chemical Neuroscience·José-Carlos Fernández-MoralesAntonio G García
Jun 20, 2012·Proceedings of the National Academy of Sciences of the United States of America·Zhongming MaJ Kevin Foskett
Jun 18, 2010·Ageing Research Reviews·Clara Hiu-Ling HungRaymond Chuen-Chung Chang
Mar 18, 2015·Biochimica Et Biophysica Acta·Daniela SalasJuan C Sáez
Jan 3, 2012·Cell Calcium·Antonio G GarcíaJavier García-Sancho
Apr 25, 2015·Neuropharmacology·Ana J Moreno-OrtegaMaría F Cano-Abad
Apr 14, 2016·Scientific Reports·Valérie VingtdeuxPhilippe Marambaud
Jul 22, 2017·Journal of Alzheimer's Disease : JAD·Anandhi IyappanUNKNOWN Alzheimer’s Disease Neuroimaging Initiative
Dec 15, 2020·Cell Calcium·Riccardo Filadi, Elisa Greotti
Aug 19, 2020·Redox Biology·Corina T Madreiter-SokolowskiMichael Ristow
Nov 9, 2020·Trends in Neurosciences·Maria Calvo-Rodriguez, Brian J Bacskai
Jan 25, 2013·Journal of Cell Science·Ute Dreses-WerringloerPhilippe Marambaud

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