Mitochondria support inositol 1,4,5-trisphosphate-mediated Ca2+ waves in cultured oligodendrocytes.

The Journal of Biological Chemistry
P B Simpson, J T Russell

Abstract

We have examined the spatial and temporal nature of Ca2+ signals activated via the phosphoinositide pathway in oligodendrocytes and the cellular specializations underlying oligodendrocyte Ca2+ response characteristics. Cultured cortical oligodendrocytes were incubated with fluo 3 or fura 2, and digital video fluorescence microscopy was used to study the effect of methacholine on [Ca2+]i. Single peaks, oscillations, and steady-state plateau [Ca2+]i elevations were evoked by increasing agonist concentration. The peaks and oscillations were found to be Ca2+ wave fronts, which propagate via distinct amplification regions in the cell where the kinetics of Ca2+ release (amplitude and rate of rise of response) are elevated. Staining with 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolecarbocyanine++ + iodide (JC-1) and 3,3'-dihexyloxacarbocyanine iodide revealed that mitochondria are found in groups of three or more in oligodendrocyte processes and that the groups are distributed with considerable distance separating them. Cross-correlation analysis showed a high degree of correlation between sites where mitochondria are present and peaks in the amplitude and rate of rise of the Ca2+ response. Intramitochondrial Ca2+ concentrat...Continue Reading

References

Jan 1, 1977·International Review of Cytology·J M Vasiliev, I M Gelfand
Jun 1, 1980·The Journal of Cell Biology·K D McCarthy, J de Vellis
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B RanschtW Seifert
Jul 1, 1995·Glia·M TakedaB Soliven
Aug 11, 1995·Cell·G HajnóczkyA P Thomas
Sep 1, 1994·Current Biology : CB·S J Smith
Sep 1, 1994·The Journal of Cell Biology·R RizzutoT Pozzan
Jun 1, 1993·Glia·C H Kastritsis, K D McCarthy
Mar 1, 1994·Journal of Neurobiology·S V YagodinJ T Russell
Jan 28, 1993·Nature·M J Berridge
Sep 1, 1995·Journal of Neuroscience Research·L A HoltzclawJ T Russell
Jan 1, 1995·Methods in Enzymology·M ReersL B Chen

❮ Previous
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Citations

Mar 29, 2001·Microscopy Research and Technique·B Soliven
May 15, 2002·Journal of Physiology and Biochemistry·A González, G M Salido
Feb 14, 2002·Biochemical Pharmacology·C Camello-AlmarazP J Camello
May 26, 1998·Brain Research. Brain Research Reviews·P B Simpson, J T Russell
Sep 23, 2003·Cellular Signalling·Antonio GonzálezJosé A Pariente
Apr 27, 2000·Trends in Biochemical Sciences·G A Rutter, R Rizzuto
Oct 1, 2003·The Journal of General Physiology·Carla M Pedrosa RibeiroRichard C Boucher
Sep 2, 1998·The EMBO Journal·L D Robb-GaspersA P Thomas
Aug 3, 2001·The EMBO Journal·P Pacher, G Hajnóczky
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A L ScottiH Reuter
Nov 6, 2008·Acta Physiologica·A SpätG Szanda
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Pál PacherGyörgy Hajnóczky
Sep 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·M HothR S Lewis
Dec 1, 2004·The Journal of Cell Biology·Nuttawut SaelimJames D Lechleiter
Aug 29, 1998·The Journal of Cell Biology·L M JohnP Camacho
Nov 18, 2000·The Journal of Physiology·G HajnóczkyP Pacher
Mar 26, 2015·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Francisco E Martin-CanoPedro J Camello
Jan 28, 2009·Neurochemistry International·Reno C Reyes, Vladimir Parpura
Jan 1, 2008·Biochemical and Biophysical Research Communications·Hiroto KumemuraMichio Sata
Apr 24, 2004·Annals of the New York Academy of Sciences·Ian J Reynolds
Dec 2, 2010·Journal of Pineal Research·Antonio GonzalezGines M Salido
Jan 12, 2013·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·John G McCarronSusan Chalmers
Aug 17, 2005·Cell Calcium·Oleg Gerasimenko, Alexei Tepikin
Jul 9, 2004·Biochimica Et Biophysica Acta·Varda Shoshan-BarmatzNoga Vardi
Jan 21, 2012·Biochemical Society Transactions·Marnie L OlsonJohn G McCarron

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