PMID: 9423287Jan 10, 1998Paper

Mitochondria transfer into mouse ova by microinjection

Transgenic Research
Carl A PinkertR J Moffatt

Abstract

A method for mitochondria isolation and interspecific transfer of mitochondria was developed in mice. Mitochondria were isolated from Mus spretus liver samples for microinjection into fertilized ova obtained from superovulated M. musculus domesticus females. Electron microscopic observations of mitochondria preparations used for microinjection demonstrated intact mitochondrial vesicles with little microsomal contamination. Species-specific nested PCR primers complementary to sequence differences in the mitochondrial DNA D-loop region revealed high rates of successful transfer of foreign mitochondria after isolation and injection into zygotes cultured through the blastocyst stage of embryonic development. Of 217 zygotes, 67 survived mitochondria injection and 23 out of 37 zygotes developed were at the blastocyst-stage of embryonic development after 4.5 days of in vitro culture. All 23 of these blastocysts contained detectable levels of foreign mitochondria. These results represent an initial step in developing a model system to study mitochondrial dynamics and development of therapeutic strategies for human metabolic diseases affected by aberrations in mitochondrial function or mutation.

Citations

Oct 2, 2001·American Journal of Medical Genetics·D C Wallace
Jul 31, 2003·Reproduction in Domestic Animals = Zuchthygiene·S Hiendleder, E Wolf
Jan 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Matthew McKenzieCarl A Pinkert
Dec 21, 2006·Pharmaceutical Research·Gerard G M D'SouzaVolkmar Weissig
Aug 30, 2007·Journal of Assisted Reproduction and Genetics·Yu-Chiao YiEsther Shih-Chu Ho
Nov 15, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Anurag Agrawal, Ulaganathan Mabalirajan
May 26, 1998·FEBS Letters·R SteinbornG Brem
Dec 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·J E SlighD C Wallace
Jun 8, 2007·Biology of Reproduction·B M ActonR F Casper
Jul 4, 2012·Nucleic Acids Research·Yi-Wei Yang, Michael D Koob
Jun 15, 2013·Obstetrics and Gynecology International·Scott Chappel
Feb 18, 2003·Molecular Reproduction and Development·Kumiko TakedaHirofumi Hanada
May 10, 2003·Proceedings. Biological Sciences·Joëlle ChatRémy J Petit
Jul 13, 2011·Pharmaceutical Research·Noha IbrahimFrédérique Weber-Lotfi
Aug 17, 2010·PLoS Genetics·Joanna PoultonIan J Holt
Aug 27, 2016·Human Reproduction Update·Pascale May-PanloupPascal Reynier
Sep 2, 1999·Nature Genetics·M J EvansE A Schon
Sep 13, 2007·Stem Cell Reviews·R Tayfur TecirliogluAlan O Trounson
Sep 22, 2011·Wiley Interdisciplinary Reviews. RNA·John W YarhamRobert W Taylor
Jul 29, 2017·Stem Cells International·Andrés CaicedoMaroun Khoury
Jul 9, 2009·Journal of Zhejiang University. Science. B·Li-ya WangChen-ming Xu
Oct 26, 2020·Journal of Inherited Metabolic Disease·James Bruce Stewart
Nov 10, 2000·Biochemical and Biophysical Research Communications·Y InokiH Endo
Jul 28, 2018·Chemical Reviews·Martin P StewartKlavs F Jensen
Mar 5, 1999·Science·D C Wallace
Jun 8, 2011·Mechanisms of Ageing and Development·Daria MileshinaFrédérique Weber-Lotfi
May 12, 2016·Cell Metabolism·Alexander N PatanananMichael A Teitell

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