PMID: 11331637May 2, 2001Paper

Mitochondrial aggregation patterns and activity in human oocytes and preimplantation embryos

Human Reproduction
M WildingG De Placido

Abstract

Mitochondria play a vital role in the metabolism of energy-containing compounds in the oocyte cytoplasm to provide adenosine trisphosphate for fertilization and preimplantation embryo development. In this study, ratiometric confocal microscopy with the mitochondrion-specific membrane potential-sensitive fluorescence dye JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide) was used to measure the activity of mitochondria in human oocytes and developing preimplantation embryos. Mitochondria in oocytes and embryos were characterized by distinct localized aggregation patterns. These patterns however did not determine localized regions of heterogeneity in mitochondrial activity. Mitochondrial activity was analysed during oocyte maturation and after fertilization. The activity of mitochondria in fresh metaphase II oocytes was negatively correlated with maternal age. This trend continued when the activity of developing embryos was analysed. Mitochondrial activity was strongly correlated with the rate of embryo development on day 3 after fertilization, but not on day 2. Partial regression analysis showed that the rate of cleavage of preimplantation embryos was more highly correlated with embryo mitochondr...Continue Reading

References

May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S T SmileyL B Chen
Aug 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·J D BiggersR P Donahue
Jun 1, 1995·Human Reproduction·Y Ménézo, B Dale
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·M K ShigenagaB N Ames
May 1, 1994·Molecular Reproduction and Development·L Janny, Y J Menezo
Nov 1, 1993·Human Reproduction·T FolgerøP Oian
Jan 1, 1995·Methods in Enzymology·M ReersL B Chen
Jan 1, 1995·Annual Review of Genetics·N G Larsson, D A Clayton
Sep 1, 1996·Molecular Reproduction and Development·L Janny, Y J Menezo
May 7, 1998·Molecular Human Reproduction·J CohenS Willadsen
Oct 2, 1998·Human Reproduction·C M WarnerC A Brenner
Oct 23, 1998·Molecular Human Reproduction·C A BrennerJ Cohen
Oct 3, 1999·Molecular Human Reproduction·J A BarrittD W Matt
Oct 21, 1999·Human Reproduction·L A Van Der WesterlakenN Naaktgeboren
Feb 17, 2000·Nature·G I PerezJ L Tilly
Sep 26, 2000·Fertility and Sterility·C A BrennerJ Cohen
Oct 21, 2000·Human Reproduction·B D Bavister, J M Squirrell
Oct 21, 2000·Human Reproduction·J A BarrittJ Cohen
Mar 1, 1995·Human Reproduction Update·B D Bavister

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Citations

Aug 30, 2007·Journal of Assisted Reproduction and Genetics·Yu-Chiao YiEsther Shih-Chu Ho
Apr 25, 2012·Journal of Assisted Reproduction and Genetics·Vitaly A KushnirWilliam Lewis
Aug 24, 2012·Journal of Assisted Reproduction and Genetics·Cui ChenGuoning Huang
Oct 12, 2012·Journal of Assisted Reproduction and Genetics·Debbie MontjeanYves Menezo
May 16, 2002·Fertility and Sterility·Rong-Hong HsiehChii-Ruey Tzeng
Mar 24, 2012·Animal : an International Journal of Animal Bioscience·J PetrF Jílek
Aug 28, 2009·Nature·Masahito TachibanaShoukhrat Mitalipov
Jun 12, 2010·Nature Protocols·Masahito TachibanaShoukhrat Mitalipov
Oct 31, 2002·Human Reproduction·Ruth RobertsKate Hardy
Jul 28, 2010·Human Reproduction·Sarah E HarrisHelen M Picton
Jul 21, 2011·Human Reproduction·Stefan CikosJuraj Koppel
May 12, 2009·Human Reproduction Update·Yi-Liang MiaoHeide Schatten
May 6, 2009·Human Reproduction Update·João Ramalho-SantosAlexandra Amaral
May 31, 2008·Molecular Human Reproduction·D H Betts, P Madan
Aug 3, 2004·Biology of Reproduction·George A ThouasGayle M Jones
Apr 22, 2005·Biology of Reproduction·George A ThouasGayle M Jones
Feb 3, 2006·Biology of Reproduction·George A ThouasGayle M Jones
Jun 12, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Wilson B M de PaulaJohn F Allen
Feb 24, 2007·The Journal of Reproduction and Development·Hiroyuki KanayaYoshihiko Hosoi
Jul 9, 2011·PloS One·L Gabriel Sanchez-PartidaJustin C St John
Oct 7, 2006·The Tohoku Journal of Experimental Medicine·Seiichiro NagaiKazuhiko Hoshi
Nov 23, 2011·Biomarkers in Medicine·Clifford D L FolmesAndre Terzic
Apr 26, 2003·Medicinski pregled·Dunja Tabs, Nebojsa Radunović
Jan 30, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Martin WildingLoredana Di Matteo
Aug 31, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Jonathan Van Blerkom
Nov 3, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Loro L Kujjo, Gloria I Perez
Feb 29, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Basudha BasuMitradas M Panicker
Dec 17, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·H M PictonE L Chambers
May 5, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Jonathan Van BlerkomPatrick Davis
Mar 29, 2014·Human Reproduction Update·Tyl H TaylorDarren K Griffin

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