Mitochondrial Alterations in Peripheral Mononuclear Blood Cells from Alzheimer's Disease and Mild Cognitive Impairment Patients

Oxidative Medicine and Cellular Longevity
A DelbarbaD Uberti

Abstract

It is well recognized that mitochondrial dysfunction contributes to neurodegeneration occurring in Alzheimer's disease (AD). However, evidences of mitochondrial defects in AD peripheral cells are still inconclusive. Here, some mitochondrial-encoded and nuclear-encoded proteins, involved in maintaining the correct mitochondria machine, were investigated in terms of protein expression and enzymatic activity in peripheral blood mononuclear cells (PBMCs) isolated from AD and Mild Cognitive Impairment (MCI) patients and healthy subjects. In addition mitochondrial DNA copy number was measured by real time PCR. We found some differences and some similarities between AD and MCI patients when compared with healthy subjects. For example, cytochrome C and cytochrome B were decreased in AD, while MCI showed only a statistical reduction of cytochrome C. On the other hand, both AD and MCI blood cells exhibited highly nitrated MnSOD, index of a prooxidant environment inside the mitochondria. TFAM, a regulator of mitochondrial genome replication and transcription, was decreased in both AD and MCI patients' blood cells. Moreover also the mitochondrial DNA amount was reduced in PBMCs from both patient groups. In conclusion these data confirmed p...Continue Reading

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Citations

May 14, 2019·The World Journal of Biological Psychiatry : the Official Journal of the World Federation of Societies of Biological Psychiatry·Piotr CzarnyPiotr Galecki
Oct 7, 2017·Journal of Alzheimer's Disease : JAD·Ayano MiseShu-Ichi Ueno
Jul 21, 2020·Medicinal Research Reviews·Elena F ShevtsovaSergey O Bachurin
May 16, 2020·Oxidative Medicine and Cellular Longevity·Alyne Mendonça Marques TonElisardo Corral Vasquez
Jan 14, 2020·Journal of Cellular and Molecular Medicine·Masoud BaghiKamran Ghaedi
Nov 2, 2019·International Journal of Molecular Sciences·Virginie Bottero, Judith A Potashkin
Nov 2, 2020·Mechanisms of Ageing and Development·G AbateD Uberti
Sep 7, 2020·Mechanisms of Ageing and Development·Ioanna Daskalaki, Nektarios Tavernarakis
May 22, 2021·Journal of Cellular and Molecular Medicine·Masoud BaghiKamran Ghaedi
Oct 8, 2020·Current Alzheimer Research·Nattaporn PakpianPrapimpun Wongchitrat
Jan 4, 2022·The International Journal of Neuropsychopharmacology·Cheng-Chen ChangHui Hua Chang

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Methods Mentioned

BETA
Immunoprecipitation
ELISA
immunoprecipitation assay
PCR

Software Mentioned

Beacon Designer
Image Studio
Graph Pad Prism Pad

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