Mitochondrial anchorage and fusion contribute to mitochondrial inheritance and quality control in the budding yeast Saccharomyces cerevisiae

Molecular Biology of the Cell
Ryo Higuchi-SanabriaLiza A Pon

Abstract

Higher-functioning mitochondria that are more reduced and have less ROS are anchored in the yeast bud tip by the Dsl1-family protein Mmr1p. Here we report a role for mitochondrial fusion in bud-tip anchorage of mitochondria. Fluorescence loss in photobleaching (FLIP) and network analysis experiments revealed that mitochondria in large buds are a continuous reticulum that is physically distinct from mitochondria in mother cells. FLIP studies also showed that mitochondria that enter the bud can fuse with mitochondria that are anchored in the bud tip. In addition, loss of fusion and mitochondrial DNA (mtDNA) by deletion of mitochondrial outer or inner membrane fusion proteins (Fzo1p or Mgm1p) leads to decreased accumulation of mitochondria at the bud tip and inheritance of fitter mitochondria by buds compared with cells with no mtDNA. Conversely, increasing the accumulation and anchorage of mitochondria in the bud tip by overexpression of MMR1 results in inheritance of less-fit mitochondria by buds and decreased replicative lifespan and healthspan. Thus quantity and quality of mitochondrial inheritance are ensured by two opposing processes: bud-tip anchorage by mitochondrial fusion and Mmr1p, which favors bulk inheritance; and qua...Continue Reading

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Citations

Nov 8, 2016·Biochimica Et Biophysica Acta. Molecular Cell Research·Markus M M BisschopsPascale Daran-Lapujade
Feb 22, 2018·Genes·Ana Rita LimaValdemar Máximo
Sep 13, 2017·Journal of Cell Science·Jan de Vries, Sven B Gould
Feb 15, 2017·Journal of Cell Science·Iuliia E KaravaevaDmitry A Knorre
Jun 16, 2017·The Journal of Cell Biology·Stefan BöcklerBenedikt Westermann
Apr 7, 2018·The Journal of Biological Chemistry·Erica A MoehleAndrew Dillin
Feb 26, 2019·The Journal of Biological Chemistry·Kun TangZhiyi Wei
Nov 7, 2019·Yeast·Bernard Dujon
Jan 23, 2020·Biological Chemistry·Till Klecker, Benedikt Westermann
Apr 23, 2020·Microorganisms·Ruei-Ching Chen, Chung-Yu Lan
Jan 24, 2018·Frontiers in Cell and Developmental Biology·Wolfgang M PerniceLiza A Pon
Apr 10, 2020·Royal Society Open Science·Yanli WangChing-Shin Chou
Aug 16, 2017·Oxidative Medicine and Cellular Longevity·Amparo Pascual-AhuirMarkus Proft
Oct 17, 2020·The Journal of Biological Chemistry·David L ProleNick S Jones
Jan 18, 2021·The Journal of Biological Chemistry·David L ProleNick S Jones
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Jul 6, 2017·Biochemical and Biophysical Research Communications·Lauren M Kraft, Laura L Lackner

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