Mitochondrial and chloroplastic targeting signals of NADP+-dependent isocitrate dehydrogenase

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
David J McKinnonGordon R Gray

Abstract

Many mitochondrial and chloroplast proteins are encoded in the nucleus and subsequently imported into the organelles via active protein transport systems. While usually highly specific, some proteins are dual-targeted to both organelles. In tobacco (Nicotiana tabacum L.), the cDNA encoding the mitochondrial isoform of NADP+-dependent isocitrate dehydrogenase (NADP+-ICDH) contains two translational ATG start sites, suggesting the possibility of tandem targeting signals. In this work, the putative mitochondrial and chloroplastic targeting signals from NADP+-ICDH were fused to a yellow fluorescent protein (YFP) reporter to generate a series of constructs and introduced into tobacco leaves by Agrobacterium-mediated transient transformation. The subsequent sub-cellular locations of the ICDH:YFP fusion proteins were then examined using confocal microscopy. Constructs predicted to be targeted to the chloroplast all localized to the chloroplast. However, this was not the case for all of the constructs that were predicted to be mitochondrial targeted. Although some constructs localized to mitochondria as expected, others appeared to be chloroplast localized. This was attributed to an additional 50 amino acid residues of the mature NADP+...Continue Reading

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Citations

Jun 12, 2012·Biochimica Et Biophysica Acta·Christopher Carrie, Ian Small
Apr 22, 2015·Protoplasma·Marina LeterrierFrancisco J Corpas
Sep 19, 2020·Journal of Experimental Botany·Francisco J CorpasJosé M Palma
Apr 12, 2020·Mitochondrion·Abir U Igamberdiev

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