Mitochondrial and nuclear DNA damage induced by curcumin in human hepatoma G2 cells

Toxicological Sciences : an Official Journal of the Society of Toxicology
Jun CaoLai-Fu Zhong

Abstract

Curcumin is extensively used as a spice and pigment and has anticarcinogenic effects that could be linked to its antioxidant properties. However, some studies suggest that this natural compound possesses both pro- and antioxidative effects. In this study, we found that curcumin induced DNA damage to both the mitochondrial and nuclear genomes in human hepatoma G2 cells. Using quantitative polymerase chain reaction and immunocytochemistry staining of 8-hydroxydeoxyguanosine, we demonstrated that curcumin induced dose-dependent damage in both the mitochondrial and nuclear genomes and that the mitochondrial damage was more extensive. Nuclear DNA fragments were also evident in comet assays. The mechanism underlies the elevated level of reactive oxygen species and lipid peroxidation generated by curcumin. The lack of DNA damage at low doses suggested that low levels of curcumin does not induce DNA damage and may play an antioxidant role in carcinogenesis. But at high doses, we found that curcumin imposed oxidative stress and damaged DNA. These data reinforce the hypothesis that curcumin plays a conflicting dual role in carcinogenesis. Also, the extensive mitochondrial DNA damage might be an initial event triggering curcumin-induced c...Continue Reading

References

Dec 15, 1992·Biochemical and Biophysical Research Communications·M HayakawaT Ozawa
Feb 1, 1991·Planta medica·H P Ammon, M A Wahl
Jan 1, 1990·Free Radical Biology & Medicine·B Bandy, A J Davison
May 31, 1988·Biochemical and Biophysical Research Communications·A M Hruszkewycz
Feb 27, 1987·Biochemical and Biophysical Research Communications·S SassaA Kappas
Jul 1, 1995·The International Journal of Biochemistry & Cell Biology·C Richter
Jan 1, 1994·Annual Review of Nutrition·J M Shoffner, D C Wallace
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·F M Yakes, B Van Houten
Mar 12, 1997·Mutation Research·N P Singh, R E Stephens
Feb 12, 1999·BMJ : British Medical Journal·M H Tattersall, H Thomas
May 13, 1999·Teratogenesis, Carcinogenesis, and Mutagenesis·J BłasiakM Wojewódzka
Sep 16, 1999·Mutation Research·D L CroteauV A Bohr
Mar 29, 2000·CA: a Cancer Journal for Clinicians·R T GreenleeP A Wingo
Jan 20, 2004·Mutation Research·K PolasaK Krishnaswamy
Aug 4, 2004·Toxicology and Applied Pharmacology·Leonard W VelsorBrian J Day
Nov 16, 2004·Archives of Biochemistry and Biophysics·Janice OnukiPaolo Di Mascio
Jun 3, 2005·Biological & Pharmaceutical Bulletin·Jong Hee SohnJae-Kwan Hwang
Jun 21, 2005·Annals of the New York Academy of Sciences·Wen-Hsiung ChanYan-Der Hsuuw
Aug 6, 2005·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R A SharmaW P Steward

❮ Previous
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Citations

Jun 27, 2009·Parasitology Research·M ShahiduzzamanA Daugschies
Feb 4, 2010·Apoptosis : an International Journal on Programmed Cell Death·Varisa PongrakhananonPithi Chanvorachote
Jul 11, 2009·The AAPS Journal·Jayaraj RavindranBharat B Aggarwal
Jun 10, 2010·World Journal of Gastroenterology : WJG·Ibrahim Abdulkarim Al Mofleh
May 25, 2011·World Journal of Gastrointestinal Pathophysiology·Sigrid A Rajasekaran
Feb 18, 2012·Future Oncology·Adeeb ShehzadYoung Sup Lee
Jul 12, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Tuan-Phat HuynhNawajes A Mandal
Jul 30, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Yan Li, Robert C G Martin
Oct 24, 2014·Expert Opinion on Drug Metabolism & Toxicology·Eleonora TurriniCarmela Fimognari
Jan 15, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Voja PavlovicJelena Jovanovic
Mar 26, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Chunpeng GaoJun Cao
Dec 19, 2012·Cancer Letters·Seung-Youn Jung, Yung-Jin Kim
May 5, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Grace G L YueClara B S Lau
Apr 24, 2009·Mutation Research·Leonardo Meneghin MendonçaLusânia Maria Greggi Antunes
Jun 25, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·So-Young ParkDonggeun Sul
Nov 21, 2007·Biochimica Et Biophysica Acta·Chiara GuidiVilberto Stocchi
Apr 17, 2007·Toxicon : Official Journal of the International Society on Toxinology·Jun CaoLai-Fu Zhong
Jan 1, 2008·BioFactors·Lucia PotenzaMarina Dachà
Jan 11, 2013·BioFactors·Adeeb ShehzadYoung Sup Lee
Nov 30, 2012·Journal of Cellular Biochemistry·Aik Kia KhawM Prakash Hande
Jan 23, 2013·BioFactors·Subash C GuptaBharat B Aggarwal
Oct 16, 2009·International Journal of Cancer. Journal International Du Cancer·Estefanía Burgos-MorónMiguel López-Lázaro
Apr 14, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Huaying XieJun Cao
Jul 15, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Lara GibelliniMarcello Pinti
Feb 25, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Chunpeng GaoJun Cao
Jan 20, 2015·Interventional Medicine & Applied Science·Muobarak Jaber Tuorkey
Apr 24, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Dimitrios StagosDimitrios Kouretas
Jun 21, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Natividad SebastiàAlegría Montoro
Jun 15, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Bin SunRebecca A Clewell
Nov 14, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sanjica RakMaja Osmak

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