PMID: 395414Jan 1, 1979

Mitochondrial and nuclear mutations that affect the biogenesis of the mitochondrial ribosomes of yeast. I. Genetics

Molecular & General Genetics : MGG
M Bolotin-Fukuhara

Abstract

We have isolated about five hundred temperature-sensitive mutants specific for the mitochondrial functions. Their growth on glycerol is defective at 36 degrees C and/or 20 degrees C. While most of the mutations were nuclearly inherited, about thirty were found to be of mitochondrial origin. 1) Four mitochondrial mutations (three cryosensitive, one thermosensitive) were localized close to chloramphenicol and erythromycin resistance loci of the mitochondrial DNA, that is in the region coding for the 23 S ribosomal RNA. One of the mutation interfered with the expression of the chloramphenicol resistance gene. 2) A dozen nuclear mutations were isolated from a strain which is labelled with mitochondrial drug resistance markers (chloramphenicol, erythromycin, and paromomycin). Among the temperature sensitive respiratory deficient mutants, we have selected the mutations that supress the resistant phenotypes. We describe two non allelic such mutations, one being cryosensitive, the other thermosensitive. Both supress the expression of the mitochondrial chloramphenicol resistance gene. The temperature sensitive growth on glycerol and the modified antibiotic phenotype segregated together as a single recessive mutation. A biochemical study...Continue Reading

References

Apr 29, 1977·Molecular & General Genetics : MGG·M Bolotin-FukuharaH Fukuhara
Feb 1, 1977·Cell·M PellegriniN Davidson
Jul 22, 1975·Biochemical and Biophysical Research Communications·J P SandersP Borst
Jul 20, 1972·Biochimica Et Biophysica Acta·L ReijndersP Borst
Feb 19, 1971·FEBS Letters·P Borst, L A Grivell

Citations

Jan 1, 1984·Molecular & General Genetics : MGG·C JulouM Bolotin-Fukuhara
Jan 1, 1984·Molecular & General Genetics : MGG·V Contamine, M Bolotin-Fukuhara
Aug 1, 1988·Molecular & General Genetics : MGG·Y W Kang, D L Miller
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P SarnowD Baltimore
Oct 11, 1988·Nucleic Acids Research·B Daignan-Fornier, M Bolotin-Fukuhara
Mar 11, 2009·DNA Repair·Nadja C de Souza-PintoVilhelm A Bohr
May 26, 2020·Nucleic Acids Research·Suhas R SeshadriFlavia Fontanesi

Related Concepts

Cell Nucleus
Genome Mapping
DNA, Fungal
DNA, Mitochondrial
Drug Resistance
Ribosomes
RNA, Fungal
15S RNA
Saccharomyces cerevisiae

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