PMID: 8609807Dec 1, 1995Paper

Mitochondrial antigen/antibody systems in primary biliary cirrhosis: revisited

Liver
P A Berg, R Klein

Abstract

Methods for the evaluation of the four antimitochondrial antibody subtypes in primary biliary cirrhosis - anti-M2, -M4, -M8, -M9 - are described. The importance of the application of different preparations for the demonstration of complement fixing antibodies and the detection of antibodies by ELISA or Western blotting is emphasized. Complement fixing antigens can be prepared by discontinuous isopynic sucrose density gradient centrifugation using mitochondrial subfractions derived with from beef heart (M2), rat liver (M4), or pig kidney (M8). Anti-M9 antibodies do not fix complement. For ELISA, the pyruvate dehydrogenase or the ATPase-associated antigen fraction (M2), the sulfite oxidase fraction (M4), and the chromatographically purified M8-fraction should be used. The same antigen fractions are suitable for Western blotting, but anti-M4 and anti-M8 by ELISA and Western blotting a purified fraction prepared from rat liver has to be applied. Correlating antimitochondrial antibody-subtypes with clinical condition and the natural course, there is convincing evidence that especially the presence of complement fixing antibodies against the subtypes M2, M4, and M8 is a reliable indicator for a more active course. Patients expressing...Continue Reading

References

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Citations

Dec 7, 2007·Apoptosis : an International Journal on Programmed Cell Death·Christoph Peter BergKirsten Lauber
Jun 1, 1997·Journal of Clinical Gastroenterology·H MörkM Scheurlen
Jun 1, 2008·Expert Opinion on Medical Diagnostics·Eirini I Rigopoulou, George N Dalekos
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Mar 1, 2013·Hepatology International·Palak J Trivedi, Sue Cullen
Mar 1, 2003·Modern Rheumatology·T KakinumaJ W Sentry
Jul 28, 2016·Expert Review of Molecular Diagnostics·Nikolaos K Gatselis, George N Dalekos

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