PMID: 12766083May 27, 2003Paper

Mitochondrial ATP-sensitive potassium channel: a novel site for neuroprotection

Investigative Ophthalmology & Visual Science
Tomofusa YamauchiAkinori Akaike

Abstract

It has been shown that bradykinin (BK) protects retinal neurons against glutamate excitotoxicity, but it was not clear how BK inhibits glutamate excitotoxicity. The purpose of this study was to investigate the effect of opening the mitochondrial adenosine triphosphate (ATP)-sensitive potassium (Mit K (ATP)) channel on glutamate excitotoxicity and the protective effect of BK using cultured retinal neurons. Primary cultures were obtained from the retina of fetal rats (gestation days 17-19). Glutamate neurotoxicity was assessed by 10-minute exposure to 1 mM glutamate followed by 1-hour incubation in glutamate-free medium, using the trypan blue exclusion method. BK, diazoxide (the opener of the Mit K (ATP) channel), 5HD, and glibenclamide (blockers of the Mit K (ATP) channel) were applied simultaneously with glutamate. Mitochondrial membrane potential was measured as the ratio of 590:527 nm fluorescence of JC-1. Cell viability was markedly reduced by 10-minute exposure to 1 mM glutamate followed by 1-hour incubation in glutamate-free medium, and glutamate induced mitochondrial depolarization of retinal neurons. BK and diazoxide protected retinal neurons against glutamate excitotoxicity and inhibited glutamate-induced mitochondrial ...Continue Reading

Citations

Mar 16, 2007·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Fang ZhouGang Hu
Apr 28, 2006·Investigative Ophthalmology & Visual Science·Steven RothVytautus Bindokas
Apr 14, 2010·International Journal of Molecular Sciences·Krisztina SzabadfiTamas Atlasz
Nov 18, 2008·Journal of Cellular and Molecular Medicine·Fang ZhouGang Hu
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Jun 5, 2013·Molecular Medicine Reports·Yi-Sheng ZhongYi-Hua Zhu
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