Mitochondrial Ca2+ Dynamics in MCU Knockout C. elegans Worms.

International Journal of Molecular Sciences
Pilar Alvarez-IlleraJavier Alvarez

Abstract

Mitochondrial [Ca2+] plays an important role in the regulation of mitochondrial function, controlling ATP production and apoptosis triggered by mitochondrial Ca2+ overload. This regulation depends on Ca2+ entry into the mitochondria during cell activation processes, which is thought to occur through the mitochondrial Ca2+ uniporter (MCU). Here, we have studied the mitochondrial Ca2+ dynamics in control and MCU-defective C. elegans worms in vivo, by using worms expressing mitochondrially-targeted YC3.60 yellow cameleon in pharynx muscle. Our data show that the small mitochondrial Ca2+ oscillations that occur during normal physiological activity of the pharynx were very similar in both control and MCU-defective worms, except for some kinetic differences that could mostly be explained by changes in neuronal stimulation of the pharynx. However, direct pharynx muscle stimulation with carbachol triggered a large and prolonged increase in mitochondrial [Ca2+] that was much larger in control worms than in MCU-defective worms. This suggests that MCU is necessary for the fast mitochondrial Ca2+ uptake induced by large cell stimulations. However, low-amplitude mitochondrial Ca2+ oscillations occurring under more physiological conditions a...Continue Reading

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Citations

Mar 27, 2021·Mechanisms of Ageing and Development·Paloma García-CasasJavier Alvarez
Apr 26, 2021·Mitochondrion·Sayantan Datta, Manish Jaiswal
Jun 3, 2021·International Journal of Molecular Sciences·David BodeFelix Hohendanner

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Methods Mentioned

BETA
PCR
electrophoresis
Chip

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NemAcquire
Metafluor

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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