Mitochondrial Ca2 + Is Related to Mitochondrial Activity and Dynamic Events in Mouse Oocytes

Frontiers in Cell and Developmental Biology
Feng WangXiang-Hong Ou

Abstract

Mitochondrial energy insufficiency is strongly associated with oocyte activation disorders. Ca2+, especially that in the mitochondrial matrix, plays a pivotal role in mitochondrial energy supplementation, but the underlying mechanisms are still only poorly understood. An encoded mitochondrial matrix Ca2+ probe (Mt-GCaMP6s) was introduced to observe mitochondrial Ca2+ ([Ca2+]m) dynamic changes during oocyte maturation and activation. We found that active mitochondria surrounding the nucleus showed a higher [Ca2+]m than those distributed in the cortex during oocyte maturation. During oocyte partheno-activation, the patterns of Ca2+ dynamic changes were synchronous in the cytoplasm and mitochondria. Such higher concentration of mitochondrial matrix Ca2+ was closely related to the distribution of mitochondrial calcium uptake (MICU) protein. We further showed that higher [Ca2+]m mitochondria around the chromosomes in oocytes might have a potential role in stimulating mitochondrial energy for calmodulin-responsive oocyte spindle formation, while synchronizing Ca2+ functions in the cytoplasm and nuclear area are important for oocyte activation.

References

Nov 5, 1974·Biochemistry·H Rottenberg, A Scarpa
Aug 11, 1995·Cell·G HajnóczkyA P Thomas
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·M J BerridgeM D Bootman
Jan 5, 2002·Journal of Bioenergetics and Biomembranes·G BáthoriM Zoratti
May 9, 2002·American Journal of Physiology. Cell Physiology·Jonathan S MarchantIan Parker
May 22, 2002·Biology of Reproduction·Ana Carla GordoRafael Fissore
Jan 23, 2004·Nature·Yuriy KirichokDavid E Clapham
Jun 7, 2005·Developmental Biology·Jean-Pierre OzilTom Ducibella
Apr 1, 2006·Seminars in Cell & Developmental Biology·Remi DumollardChristian Sardet
Jul 29, 2006·Developmental Biology·Karen Campbell, Karl Swann
Nov 11, 2006·Human Reproduction·D C Gesink LawM P Longnecker
Jan 16, 2007·Current Topics in Developmental Biology·Rémi DumollardJohn Carroll
Oct 31, 2007·Zygote : the Biology of Gametes and Early Embryos·H YazawaA Sato
Sep 16, 2008·The Journal of Biological Chemistry·Cecilia García-PérezGyörgy Csordás
Jan 27, 2009·Journal of Molecular and Cellular Cardiology·Helena M ViolaLivia C Hool
Apr 16, 2009·Biochimica Et Biophysica Acta·Elinor J Griffiths, Guy A Rutter
Jul 28, 2009·Bioscience Reports·María Carolina CassaráElvira Hinsch
Mar 9, 2010·The International Journal of Developmental Biology·Geneviève DupontLuc Leybaert
Aug 10, 2010·Nature·Fabiana PerocchiVamsi K Mootha
Aug 3, 2011·Cold Spring Harbor Protocols·Brad Chazotte
Aug 3, 2011·Journal of Visualized Experiments : JoVE·Paula Stein, Karen Schindler
Aug 5, 2011·Human Reproduction·Vivian RittenbergTarek El-Toukhy
Aug 25, 2011·Endocrinology·Kartik ShankarThomas M Badger
Aug 2, 2012·Nature Reviews. Molecular Cell Biology·Rosario RizzutoCristina Mammucari
Oct 5, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jasper AkerboomLoren L Looger
Nov 28, 2012·Nature Cell Biology·Karthik MallilankaramanMuniswamy Madesh
Oct 9, 2013·Journal of Cell Science·Takuya WakaiRafael A Fissore
Oct 30, 2013·Nutrition Reviews·Emily S JungheimMargaret M Hopeman
May 31, 2014·Biochimica Et Biophysica Acta·Deborah Naon, Luca Scorrano
Nov 26, 2014·Reproductive Biology and Endocrinology : RB&E·Heide SchattenRandall Prather
May 3, 2016·American Journal of Human Genetics·Metodi D MetodievRobert W Taylor
Jun 3, 2016·Cell Calcium·Prashant Mishra
Jun 10, 2016·Journal of Clinical Nursing·Ka-Ming ChowIrene Km Cheung
Dec 7, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Feng WangBaochang Zhu

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