Mitochondrial Ca2+ transport and permeability transition in zebrafish (Danio rerio).

Biochimica Et Biophysica Acta
Luca AzzolinPaolo Bernardi

Abstract

We have studied mitochondrial Ca(2+) transport and the permeability transition (PT) in the teleost zebrafish (Danio rerio), a key model system for human diseases. Permeabilized zebrafish embryo cells displayed a mitochondrial energy-dependent Ca(2+) uptake system that, like the Ca(2+) uniporter of mammals, was inhibited by ruthenium red. Zebrafish mitochondria underwent a Ca(2+)-dependent PT that displayed Pi-dependent desensitization by cyclosporin A, and responded appropriately to key modulators of the mammalian PT pore (voltage, pH, ubiquinone 0, dithiol oxidants and cross linkers, ligands of the adenine nucleotide translocator, arachidonic acid). Opening of the pore was documented in intact cells, where it led to death that could largely be prevented by cyclosporin A. Our results represent a necessary step toward the use of zebrafish for the screening and validation of PTP inhibitors of potential use in human diseases, as recently shown for collagen VI muscular dystrophy [Telfer et al., 2010].

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Citations

Oct 11, 2011·The Journal of Biological Chemistry·Sophia von StockumPaolo Bernardi
Feb 8, 2014·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Gina L J Galli, Jeffrey G Richards
Apr 20, 2012·Cell Calcium·Paolo Bernardi, Sophia von Stockum
Nov 17, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Reginald C AdieleCollins Kamunde
Mar 10, 2011·Mitochondrion·Salvador Uribe-CarvajalManuel Gutiérrez-Aguilar
Aug 14, 2013·Autophagy·Kim Wager, Claire Russell
Jul 28, 2016·Cell Biology International·Elis Torrezan-NitaoLuis Fernando Marques-Santos
May 25, 2018·Cell Calcium·Christopher P Baines, Manuel Gutiérrez-Aguilar
Jul 25, 2021·International Journal of Molecular Sciences·Elena IgnatievaRenata Dmitrieva

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