Mitochondrial complex II dysfunction can contribute significantly to genomic instability after exposure to ionizing radiation.

Radiation Research
Disha DayalDouglas R Spitz

Abstract

Ionizing radiation induces chronic metabolic oxidative stress and a mutator phenotype in hamster fibroblasts that is mediated by H(2)O(2), but the intracellular source of H(2)O(2) is not well defined. To determine the role of mitochondria in the radiation-induced mutator phenotype, end points of mitochondrial function were determined in unstable (CS-9 and LS-12) and stable (114) hamster fibroblast cell lines derived from GM10115 cells exposed to 10 Gy X rays. Cell lines isolated after irradiation demonstrated a 20-40% loss of mitochondrial membrane potential and an increase in mitochondrial content compared to the parental cell line GM10115. Surprisingly, no differences were observed in steady-state levels of ATP (P > 0.05). Unstable clones demonstrated increased oxygen consumption (two- to threefold; CS-9) and/or increased mitochondrial electron transport chain (ETC) complex II activity (twofold; LS-12). Using Western blot analysis and Blue Native gel electrophoresis, a significant increase in complex II subunit B protein levels was observed in LS-12 cells. Furthermore, immunoprecipitation assays revealed evidence of abnormal complex II assembly in LS-12 cells. Treatment of LS-12 cells with an inhibitor of ETC complex II (then...Continue Reading

References

Jan 1, 1979·Methods in Enzymology·R M KaschnitzH P Morris
Feb 1, 1993·Free Radical Biology & Medicine·P C PanusB A Freeman
Aug 27, 2005·Mitochondrion·Jan H SpodnikTakashi Wakabayashi
Sep 15, 2006·American Journal of Physiology. Cell Physiology·D Thor JohnsonRobert S Balaban
Mar 21, 2008·The Biochemical Journal·Disha DayalDouglas R Spitz

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Citations

Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Anita KriskoMatthew Meselson
Nov 8, 2011·Photochemistry and Photobiology·Anja HeselichPaul G Layer
Oct 14, 2010·Cancer Research·J Tyson McDonaldWilliam H McBride
Aug 8, 2013·Antioxidants & Redox Signaling·Mitchell C ColemanDouglas R Spitz
Jan 1, 2014·Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis·Jukka LuukkonenJonne Naarala
Oct 12, 2014·Journal of Radiation Research·Till DettmeringClaudia Fournier
Nov 3, 2010·International Journal of Radiation Biology·Umut AyparJanet E Baulch
Jan 20, 2016·Antioxidants·Rupak PathakNukhet Aykin-Burns
Jul 26, 2011·Mutation Research·Yaroslav Ilnytskyy, Olga Kovalchuk
Jul 27, 2015·Mitochondrion·Shama Prasada KabekkoduKapaettu Satyamoorthy
Jul 26, 2015·Free Radical Biology & Medicine·Kimberly Cramer-MoralesFrederick E Domann
Dec 24, 2010·BJU International·Ashley E RossMilena Vuica-Ross
Apr 20, 2011·Journal of Cellular Physiology·Meejeon RohSarki A Abdulkadir
Dec 18, 2013·Pigment Cell & Melanoma Research·Brian A WallSuzie Chen
Nov 26, 2015·Environmental and Molecular Mutagenesis·Janet E BaulchCharles L Limoli
Apr 3, 2016·Free Radical Biology & Medicine·Yixing WuPeter L Oliver
Oct 2, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Kimberly J KragerNukhet Aykin-Burns
Dec 17, 2014·Radiation Research·Elena K FetisovaVladimir P Skulachev
Feb 27, 2014·Radiation Research·Vijayalakshmi SridharanMarjan Boerma
Mar 8, 2013·Radiation Research·Stéphanie BlockhuysCarlos De Wagter
Feb 26, 2015·Mutagenesis·Deborah J KeszenmanJanet E Baulch
Nov 2, 2011·Free Radical Biology & Medicine·Kjerstin M OwensDouglas R Spitz
Jul 31, 2013·Free Radical Biology & Medicine·Winnie Wai-Ying Kam, Richard B Banati
Dec 21, 2011·Cancer Letters·Edouard I AzzamDebkumar Pain
Oct 23, 2016·Free Radical Biology & Medicine·Sudip BanerjeeSnehalata A Pawar
Jan 6, 2017·International Journal of Radiation Biology·Charles L Limoli
Feb 8, 2018·Journal of Radiation Research·Kasumi KawamuraJunya Kobayashi
Aug 15, 2018·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Zachary N RobinettMarlan R Hansen
Apr 8, 2010·Free Radical Research·Geou-Yarh Liou, Peter Storz
Aug 16, 2015·The Biochemical Journal·Anjaneyulu MurariNaresh Babu V Sepuri
Oct 30, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sarah Jayne Boulton, Mark A Birch-Machin
Feb 6, 2013·Physics in Medicine and Biology·H L ByrneZ Kuncic
Jun 19, 2018·Journal of Cell Communication and Signaling·Bagher FarhoodMasoud Najafi
Jul 26, 2019·Journal of Bioenergetics and Biomembranes·Galina D MironovaLudmila D Lukyanova
Apr 2, 2020·Frontiers in Pharmacology·Bjorn BaseletAn Aerts

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Datasets Mentioned

BETA
GM10115

Methods Mentioned

BETA
density gradient centrifugation
Electrophoresis
hydrogen
flow cytometry
Immunoprecipitation

Software Mentioned

GraphPad Prism

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