Mitochondrial dependent oxidative stress in cell culture induced by laser radiation at 1265 nm

Lasers in Medical Science
Yury V SaenkoAndrey Kurkov

Abstract

Photodynamic therapy is the main technique applied for surface carcinoma treatment. This technique employs singlet oxygen generated via a laser excited photosensitizer as a main damaging agent. However, prolonged sensitivity to intensive light, relatively low tissue penetration by activating light the cost of photosensitizer (PS) administration can limit photodynamic therapy applications. Early was reported singlet oxygen generation without photosensitizer induced by a laser irradiation at the wavelength of 1250-1270 nm. Here, we study the dynamics of oxidative stress, DNA damage, changes of mitochondrial potential, and mitochondrial mass induced by a laser at 1265 nm have been studied in HCT-116 and CHO-K cells. Laser irradiation of HCT-116 and CHO-K cells has induced a dose-dependent cell death via increasing intracellular reactive oxygen species (ROS) concentration, increase of DNA damage, decrease of mitochondrial potential, and reduced glutathione. It has been shown that, along with singlet oxygen generation, the increase of the intracellular ROS concentration induced by mitochondrial damage contributes to the damaging effect of the laser irradiation at 1265 nm.

References

Mar 1, 1994·Photochemistry and Photobiology·A Michaeli, J Feitelson
Mar 29, 2000·Environmental and Molecular Mutagenesis·R R TiceY F Sasaki
May 24, 2003·Biochemical and Biophysical Research Communications·Michael J Davies
Oct 17, 2003·The Journal of Physiology·Julio F Turrens
Jul 11, 2006·Biochimica Et Biophysica Acta·Dmitry B ZorovSteven J Sollott
Mar 6, 2007·Journal of Bioenergetics and Biomembranes·Russell Hilf
Aug 8, 2008·American Journal of Physiology. Cell Physiology·Dean P Jones
Mar 4, 2009·The Journal of Biological Chemistry·Diane E HandyJoseph Loscalzo
Jun 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andrew BurgessAnna Castro
Jan 18, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Zhuohui GanMarilyn P Merker
Mar 8, 2011·Biochimica Et Biophysica Acta·Fernanda RicchelliPaolo Bernardi
May 28, 2011·CA: a Cancer Journal for Clinicians·Patrizia AgostinisJakub Golab
Jun 15, 2011·Free Radical Biology & Medicine·Carla S OliveiraMauricio S Baptista
Nov 3, 2011·Photochemistry and Photobiology·François AnquezEmmanuel Courtade
Dec 18, 2013·Scientific Reports·S G SokolovskiE U Rafailov

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Citations

Sep 1, 2018·Voprosy kurortologii, fizioterapii, i lechebnoĭ fizicheskoĭ kultury·D R DolgovaA B Peskov
Nov 14, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yoshifumi KimizukaSatoshi Kashiwagi
Jun 21, 2020·Clinical Oral Investigations·Aqsa KamalA R Samsudin
Oct 20, 2020·Journal of Peptide Science : an Official Publication of the European Peptide Society·Elena IurovaYury Saenko
Jun 2, 2017·Redox Biology·Alfonso Blázquez-Castro

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