Abstract
Saturated free fatty acids (FFAs) have been implicated in the increase of oxidative stress, mitochondrial dysfunction, endoplasmic reticulum (ER) stress, autophagy, and insulin resistance (IR) observed in skeletal muscle. Previously, we have shown that palmitate-induced mitochondrial DNA (mtDNA) damage triggers mitochondrial dysfunction, mitochondrial reactive oxygen species (mtROS) production, apoptosis and IR in L6 myotubes. The present study showed that mitochondrial overexpression of human 8-oxoguanine DNA glycosylase/AP lyase (hOGG1) decreased palmitate-induced carbonylation of proteins in mitochondria. Additionally, we found that protection of mtDNA from palmitate-induced damage significantly diminished markers of both ER stress and autophagy in L6 myotubes. Moreover, we observed that the addition of ROS scavenger, N-acetylcystein (NAC), to palmitate diminished both ER stress and autophagy markers mimicking the effect of mitochondrial overexpression of hOGG1. This is the first study to show that mtDNA damage is upstream of palmitate-induced ER stress and autophagy in skeletal muscle cells.
References
Oct 16, 2004·Science·Umut OzcanGökhan S Hotamisligil
Oct 27, 2004·Nature Cell Biology·Weiming XuSalvador Moncada
Mar 11, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Hideaki KanetoYoshimitsu Yamasaki
Oct 7, 2006·Endocrinology·L I RachekG L Wilson
Sep 11, 2007·Diabetes·Eun Hee KohKi-Up Lee
Jan 12, 2008·The Journal of Clinical Investigation·Charlotte BonnardJennifer Rieusset
Dec 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Peter Kijun KimJennifer Lippincott-Schwartz
Feb 4, 2009·The Journal of Clinical Investigation·Ethan J AndersonP Darrell Neufer
Apr 16, 2009·Diabetologia·M MasiniP Marchetti
May 30, 2009·Diabetes·Andreas PeterErwin Schleicher
Jun 17, 2009·Biochimica Et Biophysica Acta·György Csordás, György Hajnóczky
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kyle L HoehnDavid E James
Mar 23, 2010·Cell·Gökhan S Hotamisligil
May 13, 2010·Biochemical and Biophysical Research Communications·Seung-Lark HwangYoung-Chae Chang
May 27, 2010·American Journal of Physiology. Endocrinology and Metabolism·Louise DeldicqueKeith Baar
Sep 30, 2010·American Journal of Physiology. Endocrinology and Metabolism·Larysa YuzefovychLyudmila Rachek
Dec 4, 2010·Science·Joshua D Rabinowitz, Eileen White
Dec 29, 2010·European Journal of Applied Physiology·Louise DeldicquePeter Hespel
Jul 14, 2011·Human Molecular Genetics·Satomi MitsuhashiIchizo Nishino
Dec 1, 2011·Endocrinology·Larysa V YuzefovychLyudmila I Rachek
Dec 23, 2011·The Biochemical Journal·Jisun LeeJianhua Zhang
Jan 24, 2013·PloS One·Larysa V YuzefovychLyudmila I Rachek
Citations
Mar 18, 2015·Molecular and Cellular Endocrinology·Ik-Rak JungYup Kang
Jun 11, 2015·International Journal of Oncology·Xu-Feng BuYu-Lan Yan
May 16, 2015·International Journal of Molecular Medicine·Chenghu HuangShuyi Cao
Mar 24, 2015·Diabetes & Metabolism·J Rieusset
Jan 16, 2015·Cell Cycle·Mauricio Menacho-MárquezJosé R Murguía
Jun 13, 2015·Journal of Ovarian Research·Perrine KafaraHubert Lincet
Sep 29, 2015·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yanggang YuanChangying Xing
Jun 24, 2015·The International Journal of Biochemistry & Cell Biology·Marjolaine A Pelissier-RotaMuriel R Jacquier-Sarlin
Nov 30, 2014·Free Radical Biology & Medicine·Tetsuo AdachiMasaru Hori
Jul 30, 2014·Diabetes·Ying LiuGary Sweeney
Oct 11, 2016·Mediators of Inflammation·Honglei GuoWei Ding
Apr 5, 2017·Nutrients·Sho HasegawaReiko Inagi
Dec 5, 2017·Physiological Reports·Myra E Woodworth-HobbsS Russ Price
Apr 4, 2018·Lipids in Health and Disease·Patrícia Ebersbach-SilvaUbiratan Fabres Machado
Oct 14, 2014·Journal of Breath Research·Paweł MochalskiAnton Amann
Jun 16, 2016·Oncotarget·Yanzhou YangWai Yee Chan
Mar 18, 2020·Endocrine Reviews·Alexis Diaz-VegasSergio Lavandero
Dec 18, 2015·Endocrinology·María E MercauCora B Cymeryng
Oct 17, 2014·Journal of Animal Physiology and Animal Nutrition·R RingseisK Eder
May 26, 2017·CNS Neuroscience & Therapeutics·Xiao-Chun DuanGang Chen
Jul 19, 2018·Frontiers in Immunology·Jin-Yuan LinLinghui Pan
Jan 5, 2018·BMC Cancer·Ana Carolina Martínez-TorresCristina Rodríguez-Padilla
Dec 14, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·O B IbitoyeT O Ajiboye
Nov 6, 2018·JCI Insight·Saurabh AggarwalSadis Matalon
Aug 18, 2018·Oxidative Medicine and Cellular Longevity·Arnold N Onyango
Jun 26, 2020·Frontiers in Physiology·Bill TachtsisNolan J Hoffman
Nov 3, 2020·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Ryosuke TatsunamiKeisuke Sato
Jul 18, 2019·DNA Repair·Harini Sampath, R Stephen Lloyd
Nov 5, 2020·International Journal of Molecular Sciences·Adriana CelesiaSonia Emanuele
Apr 2, 2021·Journal of Theoretical Biology·Irina V DokukinaEugene A Grachev