Mitochondrial DNA is critical for longevity and metabolism of transmission stage Trypanosoma brucei.

PLoS Pathogens
Caroline E DewarAchim Schnaufer

Abstract

The sleeping sickness parasite Trypanosoma brucei has a complex life cycle, alternating between a mammalian host and the tsetse fly vector. A tightly controlled developmental programme ensures parasite transmission between hosts as well as survival within them and involves strict regulation of mitochondrial activities. In the glucose-rich bloodstream, the replicative 'slender' stage is thought to produce ATP exclusively via glycolysis and uses the mitochondrial F1FO-ATP synthase as an ATP hydrolysis-driven proton pump to generate the mitochondrial membrane potential (ΔΨm). The 'procyclic' stage in the glucose-poor tsetse midgut depends on mitochondrial catabolism of amino acids for energy production, which involves oxidative phosphorylation with ATP production via the F1FO-ATP synthase. Both modes of the F1FO enzyme critically depend on FO subunit a, which is encoded in the parasite's mitochondrial DNA (kinetoplast or kDNA). Comparatively little is known about mitochondrial function and the role of kDNA in non-replicative 'stumpy' bloodstream forms, a developmental stage essential for disease transmission. Here we show that the L262P mutation in the nuclear-encoded F1 subunit γ that permits survival of 'slender' bloodstream for...Continue Reading

References

Feb 15, 1977·European Journal of Biochemistry·M Klingenberg, H Rottenberg
Dec 1, 1976·Experimental Parasitology·W J Herbert, W H Lumsden
Apr 11, 1991·Molecular and Biochemical Parasitology·E J BienenA B Clarkson
Sep 1, 1986·Molecular and Biochemical Parasitology·J E FeaginK Stuart
May 15, 1973·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·I W Flynn, I B Bowman
Dec 1, 1994·Current Opinion in Structural Biology·J E Walker
Aug 15, 1993·European Journal of Biochemistry·E J BienenA B Clarkson
May 1, 1993·The Journal of Eukaryotic Microbiology·A A DivoA C Sartorelli
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·J J Van HellemondA G Tielens
Jul 4, 2002·The Journal of Biological Chemistry·Natacha Bochud-Allemann, André Schneider
Jul 16, 2002·International Journal for Parasitology·Achim SchnauferKen Stuart
May 27, 2003·Apoptosis : an International Journal on Programmed Cell Death·J D LyA Lawen
Mar 24, 2004·Biochemical Pharmacology·Laurent AzemaMichael P Barrett
Jan 14, 2005·The Journal of Biological Chemistry·Susanne W H van WeeldenAloysius G M Tielens
Jan 25, 2005·The Journal of Biological Chemistry·Nadia LamourFrédéric Bringaud
Jun 1, 2005·Trends in Parasitology·Filip ClaesBruno Maria Goddeeris
Sep 15, 2005·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Z K NjiruW C Gibson
Oct 14, 2005·Advances in Microbial Physiology·Jaap J van HellemondAloysius G M Tielens
Jan 5, 2006·Genes & Development·Wei-Xing Zong, Craig B Thompson
May 26, 2009·Molecular and Biochemical Parasitology·Claudia ColasanteFrank Voncken
Jun 27, 2009·PLoS Computational Biology·Nicholas J SavillSarah E Reece
Aug 31, 1912·British Medical Journal·W Yorke, B Blacklock
Aug 31, 2010·Trends in Parasitology·Achim Schnaufer
Sep 21, 2010·Molecular and Biochemical Parasitology·Gabriela Schumann BurkardIsabel Roditi
Oct 14, 2010·PLoS Computational Biology·Martin R MillerNicholas J Savill

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Citations

Apr 11, 2020·PLoS Neglected Tropical Diseases·Wandayi Emmanuel AmlabuTheresa Manful Gwira
Feb 23, 2020·Journal of Cell Science·Jonathan C MillerMichele M Klingbeil
Mar 14, 2020·Nature Communications·Olivia J S MacleodMark Carrington
Feb 6, 2020·Trends in Parasitology·Balázs SzöőrKeith R Matthews
Aug 17, 2021·Microbial Genomics·Guy OldrieveKeith R Matthews
Aug 5, 2021·Annual Review of Microbiology·Keith R Matthews
Feb 2, 2022·ELife·Keith R Matthews, Stephen Larcombe

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Methods Mentioned

BETA
PCR
flow cytometry
FCS
X-ray
fluorescence microscopy

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