Mitochondrial dysfunction in patients with severe sepsis: an EPR interrogation of individual respiratory chain components

Biochimica Et Biophysica Acta
Dimitri A SvistunenkoChris E Cooper

Abstract

Electron paramagnetic resonance (EPR) spectra of complex biological systems contain information about the paramagnetic centres present. Retrieving such information is important since paramagnetic species are common intermediates of all redox reactions in both normal and abnormal metabolism. However, it is often difficult to determine the nature and content of all paramagnetic species present because the EPR signals from individual centres overlap. Here, we apply our deconvolution method based on spectra subtraction with variable coefficient to quantify individual paramagnetic components of human muscle biopsies taken from critically ill patients with severe sepsis. We use low temperature EPR spectroscopy to identify and quantify nine different paramagnetic species in the tissue. These include the majority of the mitochondrial iron-sulfur centres and the first in vivo report of a mitochondrial radical assigned to a spin-coupled pair of semiquinones (SQ*-SQ*). We have previously demonstrated in these same muscle biopsies that biochemical assays of mitochondrial dysfunction correlate with clinical outcomes (D. Brealey, M. Brand, I. Hargreaves, S. Heales, J. Land, R. Smolenski, N.A. Davies, C.E. Cooper, M. Singer, Association betwe...Continue Reading

References

Nov 1, 1976·Archives of Biochemistry and Biophysics·W J IngledewT Ohnishi
Oct 20, 1975·Biochimica Et Biophysica Acta·D L Williams-Smith, P J Morrison
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·F J RuzickaR H Sands
Feb 7, 1977·Biochimica Et Biophysica Acta·S P AlbrachtB V Swol
Oct 1, 1985·Critical Care Medicine·W A KnausJ E Zimmerman
Dec 31, 1982·Biochimica Et Biophysica Acta·H Beinert, S P Albracht
Sep 1, 1995·European Journal of Biochemistry·S AnemüllerG Schäfer
Feb 1, 1994·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·H M SwartzT Walczak
Jan 1, 1993·Journal of Neural Transmission. Parkinson's Disease and Dementia Section·L Zecca, H M Swartz
Dec 1, 1995·Physics in Medicine and Biology·S L Breen, J J Battista
Jul 1, 1996·Free Radical Research·D P Naughton, M C Symons
Nov 12, 1996·Biochemical and Biophysical Research Communications·J K ShergillA H Schapira
Mar 14, 1997·The Journal of Biological Chemistry·D A SvistunenkoM T Wilson
Jun 19, 1998·Biochimica Et Biophysica Acta·T Ohnishi
Jul 8, 1999·Cancer Letters·M SokM Schara
Apr 11, 2001·Biochimica Et Biophysica Acta·D A Svistunenko
Nov 5, 2002·Biophysical Journal·Dimitri A SvistunenkoChris E Cooper
May 15, 2003·The Journal of Biological Chemistry·Jonathan P McHughSimon C Andrews
Sep 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·B COMMONER, J L TERNBERG
Nov 8, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·David BrealeyMervyn Singer
Jan 15, 2004·Journal of the American Chemical Society·Simon A MaloneGeoffrey R Moore

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Citations

Jul 8, 2011·Human Genetics·Germaine EscamesDarío Acuña-Castroviejo
Apr 7, 2010·Wiener medizinische Wochenschrift·Martina Wendel, Axel R Heller
Oct 5, 2010·Cardiovascular Research·Anje Cauwels, Peter Brouckaert
Sep 18, 2010·International Journal of Clinical Practice·M E BernalL Montagnier
Feb 9, 2012·American Journal of Respiratory and Critical Care Medicine·Nancy Chou MacGarveyClaude A Piantadosi
Oct 13, 2006·Malaria Journal·Ian A ClarkWilliam B Cowden
Sep 7, 2006·Critical Care : the Official Journal of the Critical Care Forum·Alessandro Protti, Mervyn Singer
Feb 6, 2010·Critical Care Medicine·Leigh Ann Callahan, Gerald S Supinski
Aug 15, 2009·Neurochemical Research·Felipe Dal-PizzolEmilio L Streck
May 23, 2012·Critical Care Medicine·Lonneke SmedingClaudia C dos Santos
Sep 15, 2007·Critical Care Medicine·Alessandro ProttiMervyn Singer
Sep 22, 2007·Critical Care Medicine·Gianni BioloMarcello De Cicco
May 10, 2008·Free Radical Biology & Medicine·Martyna ElasStefan Chlopicki
Feb 24, 2007·Experimental Neurology·Fabrice RannouMarie-Agnès Giroux-Metges
Jun 30, 2015·Free Radical Biology & Medicine·Anup SrivastavaPraveen Mannam
May 17, 2008·Biochimica Et Biophysica Acta·Jane E Carré, Mervyn Singer
Oct 30, 2007·Biochimica Et Biophysica Acta·Tatiana El-BachaAndrea T Da Poian
Apr 21, 2012·Journal of the American Chemical Society·Brandon J ReederMichael T Wilson
Nov 26, 2010·Journal of the American Chemical Society·Matthew K ThompsonDimitri A Svistunenko
Apr 6, 2018·Chemical Science·Ma Teresa Pellicer MartinezNick E Le Brun
Feb 23, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Wilfred R Hagen
Oct 21, 2006·Current Opinion in Clinical Nutrition and Metabolic Care·Thierry LemineurJean-Charles Preiser
Sep 1, 2009·Metallomics : Integrated Biometal Science·Wilfred R Hagen
Apr 5, 2019·Chemistry : a European Journal·Amanda K ChaplinJonathan A R Worrall
Feb 4, 2012·Shock·Lonneke SmedingMartin C J Kneyber
Jul 7, 2018·EMBO Molecular Medicine·Lise Van WyngeneClaude Libert
Sep 19, 2019·ELife·Ma Teresa Pellicer MartinezNick E Le Brun
Jan 17, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Marina LučićJonathan A R Worrall
Oct 27, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kiichi Nakahira, Augustine M K Choi

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