Mitochondrial dysfunction induces NLRP3 inflammasome activation during cerebral ischemia/reperfusion injury

Journal of Neuroinflammation
Zhe GongYing Peng

Abstract

Nod-like receptor protein 3 (NLRP3) inflammasome is a crucial factor in mediating inflammatory responses after cerebral ischemia/reperfusion (I/R), but the cellular location of NLRP3 inflammasome in cerebral I/R has yet come to a conclusion, and there is still no specific evidence to state the relationship between mitochondria and the NLRP3 inflammasome in cerebral I/R. In the present study, we detected the cellular localization of NLRP3 inflammasomes in a transient middle cerebral artery occlusion (tMCAO) rat model and a transwell co-culture cell system under oxygen-glucose deprivation/reoxygenation (OGD/R) conditions. Then, we investigated the relationship between mitochondrial dysfunction and the activation of NLRP3 inflammasomes in different cell types after OGD/R and cerebral I/R injury. Our results showed that NLRP3 inflammasomes were first activated in microglia soon after cerebral I/R injury onset and then were expressed in neurons and microvascular endothelial cells later, but they were mainly in neurons. Furthermore, mitochondrial dysfunction played an important role in activating NLRP3 inflammasomes in microglia after OGD/R, and mitochondrial protector could inhibit the activation of NLRP3 inflammasomes in cerebral I...Continue Reading

References

Mar 14, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Anne-Claire LukaszeviczBrigitte Onténiente
May 3, 2003·Methods : a Companion to Methods in Enzymology·Violetta Zujovic, Veronique Taupin
Apr 19, 2007·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Adam DenesStuart M Allan
Jun 30, 2007·Cell Death and Differentiation·V PétrilliJ Tschopp
Dec 11, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Denise P AbulafiaW Dalton Dietrich
Jan 12, 2010·Current Opinion in Immunology·Eicke Latz
Mar 5, 2010·European Journal of Immunology·Veit Hornung, Eicke Latz
Mar 23, 2010·Cell·Kate Schroder, Jurg Tschopp
Jul 20, 2010·Cell Host & Microbe·Mohamed Lamkanfi, Vishva M Dixit
Oct 20, 2010·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Jin-rui PanYi-dong Wang
Dec 3, 2010·Nature·Rongbin ZhouJürg Tschopp
Aug 4, 2012·Journal of Leukocyte Biology·Anna Rubartelli
Jan 16, 2014·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Fan YangFan Yi
May 20, 2014·Experimental Eye Research·Qinxiang ZhengWei Chen
Oct 6, 2014·Current Opinion in Immunology·Hajime KonoEicke Latz
Dec 17, 2014·Trends in Molecular Medicine·Prajwal GurungThirumala-Devi Kanneganti
Mar 31, 2015·Journal of the Neurological Sciences·Guadalupe PalenciaCristina Trejo-Solís
Nov 4, 2015·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Wei ChenSuxia Shao
Mar 22, 2016·CNS & Neurological Disorders Drug Targets·Zhiliang GuoXinfeng Liu
Sep 1, 2016·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Li GaoYansheng Li
Sep 8, 2016·Archives of Pharmacal Research·Je-Wook Yu, Myung-Shik Lee
Nov 22, 2016·Chemico-biological Interactions·Aline M LucasHeberty T Facundo
Nov 18, 2017·Stroke; a Journal of Cerebral Circulation·Yicong ChenMoshe Gavish

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Citations

Oct 28, 2019·Cancers·Eleanna KaffeVassilis Aidinis
Jul 16, 2020·CNS Neuroscience & Therapeutics·Hong-Guang DingHong-Ke Zeng
Jan 11, 2020·Frontiers in Neurology·Aeja JacksonAli Keshavarzian
Jun 28, 2020·Translational Stroke Research·Wei ChenAlireza Sarkaki
Mar 18, 2019·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Jie LuoAnumantha G Kanthasamy
May 21, 2019·Free Radical Research·Pietro ScicchitanoMarco Matteo Ciccone
Aug 8, 2020·Klinicheskaia laboratornaia diagnostika·A V LugovayaA V Artemova
Apr 25, 2019·EMBO Molecular Medicine·Sofie VoetGeert van Loo
Jun 26, 2020·Frontiers in Cellular Neuroscience·Ya-Shuo FengFeng Zhang
Jul 9, 2020·Cells·Ádám MészárosIstván A Krizbai
Oct 18, 2020·Biomolecules·Bianca VezzaniCarlotta Giorgi
Jan 19, 2021·Medicinal Research Reviews·Carolina PellegriniVincenzo Calderone
Mar 24, 2021·Histochemistry and Cell Biology·Sanjana Mahadev BhatChandrashekhar Charavaryamath
Jul 19, 2020·Journal of Neuroimmunology·Narayanappa AmrutaGregory Bix
Apr 4, 2021·Antioxidants·Eduardo Felipe Alves Fernandes, Dennis Özcelik

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Methods Mentioned

BETA
flow cytometry
PCR
ELISAs
ELISA
transfection

Software Mentioned

Pro Plus
Image
SPSS

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