Mitochondrial electron transport inhibition in full genomic hepatitis C virus replicon cells is restored by reducing viral replication

Liver International : Official Journal of the International Association for the Study of the Liver
Mie AndoIsao Sakaida

Abstract

Hepatitis C virus (HCV) core protein has been shown to inhibit mitochondrial electron transport and to increase reactive oxygen species (ROS) in vitro and in vivo. The aim of this study was to investigate whether inhibiting HCV replication could restore the mitochondrial redox state and electron transport activity. We measured ROS, mitochondrial reduced glutathione content, and mitochondrial complex I, II, III and IV activities and protein expression in full genomic HCV replicon cells and cured cells that had been prepared by eliminating HCV RNA from replicon cells by interferon (IFN)-alpha treatment. Cured cells had significantly lower ROS production and greater mitochondrial glutathione content than replicon cells. Complete inhibition of HCV replication by IFN-alpha restored complex I and IV activities by 20-30% (P<0.01) and complex I expression (P<0.05). Treatment with fluvastatin, one of the 3-hydroxy-3-methylglutaryl co-enzyme A reductase inhibitors, which is known to have anti-HCV activity, partially inhibited core protein expression and restored complex I activity in full genomic HCV replicon cells to a lesser degree (P<0.05). Our results show that the mitochondrial redox state and electron transport activity can be rest...Continue Reading

References

Mar 1, 1985·Archives of Biochemistry and Biophysics·J F TurrensA L Lehninger
Nov 1, 1980·The Biochemical Journal·J F Turrens, A Boveris
Jun 27, 2000·Gastroenterology·G L Davis
Jul 29, 2000·Methods in Enzymology·S A SusinG Kroemer
Sep 27, 2002·The New England Journal of Medicine·Michael W FriedJian Yu
Oct 31, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Leonard B Seeff
Nov 7, 2002·The Journal of Biological Chemistry·Ken-ichi SetsukinaiTetsuo Nagano
Sep 27, 2003·Circulation Research·Steven P JonesEduardo Marbán
Dec 6, 2003·The Journal of Antimicrobial Chemotherapy·Keisuke Hino, Kiwamu Okita
Mar 16, 2005·Biochemical and Biophysical Research Communications·Masanori IkedaNobuyuki Kato
Jun 27, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Masanori IkedaNobuyuki Kato
Jan 31, 2007·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Laetitia FartouxLawrence Serfaty
Mar 30, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jacqueline G O'LearyRaymond T Chung

❮ Previous
Next ❯

Citations

Feb 9, 2010·QJM : Monthly Journal of the Association of Physicians·J H PatelS D Taylor-Robinson
Jan 30, 2013·Viruses·Alexander V IvanovSergey N Kochetkov
Feb 5, 2014·Laboratory Investigation; a Journal of Technical Methods and Pathology·Sandra Pisonero-VaqueroSonia Sánchez-Campos
Dec 18, 2013·Archives of Virology·C Claus, U G Liebert
Oct 12, 2013·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Keisuke HinoSohji Nishina
Mar 14, 2013·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Li-Jie SunBing-Zhu Yan
Dec 29, 2013·Oxidative Medicine and Cellular Longevity·Mario ArcielloClara Balsano
Aug 21, 2013·Current Pathobiology Reports·Ting Wang, Steven A Weinman
Jul 20, 2019·Reviews in Medical Virology·Changbo QuQiuwei Pan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Related Papers

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Jinah ChoiJing-Hsiung Ou
Journal of Clinical Gastroenterology
Masaaki KorenagaSteven A Weinman
Nature Reviews. Molecular Cell Biology
Richard J Youle, Derek Narendra
© 2022 Meta ULC. All rights reserved