Mitochondrial free fatty acid β-oxidation supports oxidative phosphorylation and proliferation in cancer cells

The International Journal of Biochemistry & Cell Biology
Sara Rodríguez-EnríquezRafael Moreno-Sánchez

Abstract

Oxidative phosphorylation (OxPhos) is functional and sustains tumor proliferation in several cancer cell types. To establish whether mitochondrial β-oxidation of free fatty acids (FFAs) contributes to cancer OxPhos functioning, its protein contents and enzyme activities, as well as respiratory rates and electrical membrane potential (ΔΨm) driven by FFA oxidation were assessed in rat AS-30D hepatoma and liver (RLM) mitochondria. Higher protein contents (1.4-3 times) of β-oxidation (CPT1, SCAD) as well as proteins and enzyme activities (1.7-13-times) of Krebs cycle (KC: ICD, 2OGDH, PDH, ME, GA), and respiratory chain (RC: COX) were determined in hepatoma mitochondria vs. RLM. Although increased cholesterol content (9-times vs. RLM) was determined in the hepatoma mitochondrial membranes, FFAs and other NAD-linked substrates were oxidized faster (1.6-6.6 times) by hepatoma mitochondria than RLM, maintaining similar ΔΨm values. The contents of β-oxidation, KC and RC enzymes were also assessed in cells. The mitochondrial enzyme levels in human cervix cancer HeLa and AS-30D cells were higher than those observed in rat hepatocytes whereas in human breast cancer biopsies, CPT1 and SCAD contents were lower than in human breast normal tis...Continue Reading

References

Oct 1, 1976·FEBS Letters·K E Akerman, M K Wikström
Aug 1, 1992·Archives of Biochemistry and Biophysics·G G ChangC L Meng
Jan 1, 1985·Archives of Biochemistry and Biophysics·G W Svanas, H Weiner
May 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·L R OrrickH Busch
Mar 1, 1984·Analytical Biochemistry·R W Moreadith, G Fiskum
Jun 1, 1995·Archives of Biochemistry and Biophysics·Rafael Moreno-SánchezN Corona
Jul 27, 1994·Molecular and Cellular Biochemistry·D A BriscoeJ K Kelleher
Aug 15, 1993·Archives of Biochemistry and Biophysics·D J Dietzen, E J Davis
Jul 26, 1996·Biochemical Pharmacology·J A KennedyJohn D Horowitz
Feb 23, 2000·Archives of Biochemistry and Biophysics·Sara Rodríguez-EnríquezRafael Moreno-Sánchez
Apr 12, 2001·European Journal of Biochemistry·Sara Rodríguez-EnríquezRafael Moreno-Sánchez
Jun 6, 2003·Biochemical Pharmacology·Alvaro Marín-HernándezRafael Moreno-Sánchez
Sep 16, 2003·Proteomics·Paul C HerrmannEmanuel F Petricoin
Aug 10, 1956·Science·O WARBURG
Dec 31, 2003·Biochemical and Biophysical Research Communications·Xin Lin Zu, Michael Guppy
Feb 12, 2004·Cancer Research·Rodrigue RossignolRoderick A Capaldi
Jun 25, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Adrián Báez-RuizMauricio Díaz-Muñoz
Feb 14, 2006·European Journal of Cardiovascular Nursing : Journal of the Working Group on Cardiovascular Nursing of the European Society of Cardiology·Sally Inglis, Simon Stewart
Sep 22, 2006·Biochimica Et Biophysica Acta·Ferdinando ChiaradonnaLilia Alberghina
Feb 17, 2007·The FEBS Journal·Rafael Moreno-SánchezEmma Saavedra
Apr 21, 2007·Cardiovascular Drug Reviews·Houman AshrafianMichael P Frenneaux
Aug 28, 2007·International Journal of Cancer. Journal International Du Cancer·Jian-Feng LinFu-Kun Zhao
Feb 7, 2008·Cancer Biology & Therapy·Paola MazzarelliLuigi Giusto Spagnoli
Apr 17, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Johannes A MayrBarbara Kofler
Jan 6, 2009·Molecular Nutrition & Food Research·Sara Rodríguez-EnríquezRafael Moreno-Sánchez
Jun 30, 2009·Journal of Cellular Biochemistry·Juan Carlos Gallardo-PérezV Maldonado
Aug 20, 2009·Archives of Biochemistry and Biophysics·Michael J MacDonaldMindy A Kendrick
Dec 30, 2009·The Journal of Clinical Investigation·Ismael SamudioMichael Andreeff
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Apr 2, 2011·Biochimica Et Biophysica Acta·Inthrani R IndranCatherine Brenner

Citations

Jan 17, 2016·Biochimica Et Biophysica Acta·Juan García-RincónCarmen Beltrán
Oct 6, 2015·Biochimica Et Biophysica Acta·Ileana Hernández-ReséndizSara Rodríguez-Enríquez
Jan 15, 2017·Molecular and Cellular Endocrinology·Paula Saavedra-GarcíaEric W-F Lam
Jan 18, 2018·International Journal of Epidemiology·Benjamin ElsworthTom R Gaunt
Sep 7, 2019·Journal of Cellular Physiology·Oya AltinokZulfiya Orynbayeva
Sep 17, 2017·Oncotarget·Thomas W FlaigIsabel R Schlaepfer
Apr 19, 2017·Oncotarget·Harri M ItkonenIan G Mills
May 1, 2018·Current Medicinal Chemistry·Camille AttanéCatherine Muller
Dec 2, 2016·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Benjamin D HopkinsLewis C Cantley
Feb 17, 2019·Archives of Pharmacal Research·Min-Kyue Shin, Jae-Ho Cheong
Aug 7, 2017·Oxidative Medicine and Cellular Longevity·Andre KoitTuuli Kaambre
Feb 17, 2019·Breast Cancer Research and Treatment·Hua ZhaoWong-Ho Chow
May 20, 2016·Molecular Cancer Therapeutics·Zheqiong TanYa Cao
Dec 1, 2020·Frontiers in Oncology·Martin BöttcherDimitrios Mougiakakos
Dec 29, 2020·Frontiers in Oncology·Wenjun WangJiuwei Cui

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