Mitochondrial function is altered in articular chondrocytes of an endemic osteoarthritis, Kashin-Beck disease

Osteoarthritis and Cartilage
J T LiuY D Bai

Abstract

Kashin-Beck disease (KBD) is an endemic degenerative osteoarthritis (OA) associated with extracellular matrix degradation and chondrocyte necrosis in the articular and growth plate cartilage. The role of mitochondria in degenerative diseases is widely recognized but its function in KBD is unknown. The aim of this investigation was to evaluate mitochondrial function to understand the mitochondria-mediated caspase activation and apoptosis in adult KBD chondrocytes. Mitochondrial function was evaluated by analyzing the activities of respiratory chain enzyme complexes and citrate synthase (CS), intracellular adenosine triphosphate (ATP) contents, as well as changes in mitochondrial membrane potential (DeltaPsim). Apoptotic cell death was evaluated by analyzing the cytochrome c release from mitochondria to the cytosol, caspase-9 and 3 activities, and the apoptosis rate of KBD articular chondrocytes. Activities of complexes II, III, IV and V were reduced in KBD articular chondrocytes compared with cells from normal controls. However, the mitochondrial mass was increased in KBD samples. Cultured KBD chondrocytes had a reduction of cellular ATP levels and contained a higher proportion of cells with de-energized mitochondria. Mitochondr...Continue Reading

References

Jan 1, 1983·Toxicon : Official Journal of the International Society on Toxinology·J G Pace
Jan 1, 1994·Cardiovascular Research·H M PiperB Siegmund
Aug 30, 1996·The Journal of Biological Chemistry·A KrippnerB M Babior
Jan 16, 1999·Free Radical Biology & Medicine·S M CardosoR Oliveira
Jul 14, 1999·Arthritis and Rheumatism·H A Kim, Y W Song
Aug 3, 2001·International Orthopaedics·T Yamamuro
Aug 3, 2001·International Orthopaedics·J L PasteelsN Perlmutter
Dec 14, 2001·Proceedings of the National Academy of Sciences of the United States of America·A AlmeidaS Moncada
Feb 16, 2002·The Science of the Total Environment·Jian'an TanLinsheng Yang
Mar 13, 2003·Arthritis and Rheumatism·Emilia ManeiroFrancisco J Blanco
Apr 13, 2004·Arthritis and Rheumatism·Kristen JohnsonRobert Terkeltaub
Jun 30, 2004·Diabetes & Metabolism·B FromentyD Pessayre
Aug 3, 2004·Science·Douglas R Green, Guido Kroemer
Mar 4, 2005·The International Journal of Biochemistry & Cell Biology·Bradford W Gibson
Aug 27, 2005·Mitochondrion·Robert TerkeltaubSoumitra Ghosh
Aug 27, 2005·Mitochondrion·Francisco J BlancoEmilia Maneiro
May 9, 2006·Osteoarthritis and Cartilage·M J López-ArmadaF J Blanco
Jul 4, 2006·Lancet·Anthony H V Schapira
Feb 17, 2007·Neuroscience·E Trushina, C T McMurray
Apr 28, 2007·Circulation Research·Sean M Davidson, Michael R Duchen
Jul 25, 2009·The Biochemical Journal·Frederick P BellingerMarla J Berry

❮ Previous
Next ❯

Citations

Jun 29, 2011·Current Opinion in Rheumatology·Richard F Loeser
May 23, 2015·International Journal of Molecular Sciences·Xi WangXiong Guo
Jun 20, 2012·Expert Opinion on Drug Discovery·J Paul RobinsonVincent Jo Davisson
Jan 22, 2013·Mitochondrion·María J López-ArmadaMarta N Valcárcel-Ares
Aug 30, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Katherine J WolffJames A Martin
Oct 16, 2015·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·Y WenF Zhang
Apr 25, 2016·Functional & Integrative Genomics·Yan WenFeng Zhang
Jul 19, 2016·Cell Biology International·Jidong YanShemin Lu
Oct 26, 2016·Journal of Pineal Research·Azam HosseinzadehSaeed Mehrzadi
Apr 7, 2017·Nature Reviews. Rheumatology·Ali MobasheriUrsula Fearon
Aug 12, 2016·Physiological Reviews·Michael J Berridge
Apr 21, 2020·International Journal of Nanomedicine·Hyo Jung ShinDong Woon Kim
Apr 25, 2020·Frontiers in Physiology·Devasena Ponnalagu, Harpreet Singh
May 30, 2020·Cellular and Molecular Life Sciences : CMLS·Yuzhe HeLidong Wu

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.