PMID: 7539612Jun 1, 1995Paper

Mitochondrial gene expression in small intestinal epithelial cells

The Biochemical Journal
T P MayallA J Macpherson

Abstract

Most mitochondrial genes are transcribed as a single large transcript from the heavy strand of mitochondrial DNA, and are subsequently processed into the proximal mitochondrial (mt) 12 S and 16 S rRNAs, and the more distal tRNAs and mRNAs. We have shown that in intestinal epithelial biopsies the steady-state levels of mt 12 S and 16 S rRNA are an order of magnitude greater than those of mt mRNAs. Fractionation of rat small intestinal epithelial cells on the basis of their maturity has shown that the greatest ratios of 12 S mt rRNA/cytochrome b mt mRNA or 12 S mt rRNA/cytochrome oxidase I mt mRNA are found in the surface mature enterocytes, with a progressive decrease towards the crypt immature enteroblasts. Cytochrome b and cytochrome oxidase I mt mRNA levels are relatively uniform along the crypt-villus axis, but fractionation experiments showed increased levels in the crypt base. The levels of human mitochondrial transcription factor A are also greater in immature crypt enteroblasts compared with mature villus enterocytes. These results show that the relative levels of mt rRNA and mRNA are distinctly regulated in intestinal epithelial cells according to the crypt-villus position and differentiation status of the cells, and th...Continue Reading

Citations

Jan 1, 1997·Annual Review of Biochemistry·G S Shadel, D A Clayton
Dec 13, 2006·Journal of Gastroenterology·Lyrissa Baksheev, Peter J Fuller
Dec 14, 2002·American Journal of Respiratory and Critical Care Medicine·Hagir B SulimanClaude A Piantadosi
Nov 10, 2000·Biochimica Et Biophysica Acta·K H KainN K Herzog

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