Mitochondrial Genome Maintenance 1 (Mgm1) Protein Alters Membrane Topology and Promotes Local Membrane Bending

Journal of Molecular Biology
Jarungjit RujiviphatG Angus McQuibban

Abstract

Large GTPases of the dynamin superfamily promote membrane fusion and division, processes that are crucial for intracellular trafficking and organellar dynamics. To promote membrane scission, dynamin proteins polymerize, wrap around, and constrict the membrane; however, the mechanism underlying their role in membrane fusion remains unclear. We previously reported that the mitochondrial dynamin-related protein mitochondrial genome maintenance 1 (Mgm1) mediates fusion by first tethering opposing membranes and then undergoing a nucleotide-dependent structural transition. However, it is still unclear how Mgm1 directly affects the membrane to drive fusion of tethered membranes. Here, we show that Mgm1 association with the membrane alters the topography of the membrane, promoting local membrane bending. We also demonstrate that Mgm1 creates membrane ruffles resulting in the formation of tubular structures on both supported lipid bilayers and liposomes. These data suggest that Mgm1 membrane interactions impose a mechanical force on the membrane to overcome the hydrophilic repulsion of the phospholipid head groups and initiate the fusion reaction. The work reported here provides new insights into a possible mechanism of Mgm1-driven mito...Continue Reading

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Citations

Jun 17, 2015·Journal of Molecular Biology·Sandra G Schrempp, Martin van der Laan
Sep 11, 2016·Biochimica Et Biophysica Acta. Molecular Cell Research·Heike RampeltNikolaus Pfanner
May 18, 2016·Journal of Cell Science·Thomas MacVicar, Thomas Langer
Apr 2, 2020·Biological Chemistry·Siavash Khosravi, Max E Harner
Dec 4, 2016·Biochemical Society Transactions·Hakjoo Lee, Yisang Yoon
Feb 12, 2020·Proceedings of the National Academy of Sciences of the United States of America·Liming YanJunjie Hu
Jan 17, 2019·F1000Research·Mickael M Cohen, David Tareste
Jun 29, 2021·Frontiers in Neurology·Valentina Del Dotto, Valerio Carelli

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