PMID: 11332897May 3, 2001Paper

Mitochondrial localization and photodamage during photodynamic therapy with tetraphenylporphines

Journal of Photochemistry and Photobiology. B, Biology
E WeizmanZ Malik

Abstract

The subcellular localization sites of TPPS4 and TPPS1 and the subsequent cellular site damage during photodynamic therapy were investigated in CT-26 colon carcinoma cells using spectroscopic and electron microscopy techniques. The association of both porphyrins with the mitochondria was investigated and the implications of this association on cellular functions were determined. Spectrofluorescence measurements showed that TPPS4 favors an aqueous environment, while TPPS1 interacts with lipophilic complexes. The subcellular localization sites of each sensitizer were determined using spectral imaging. Mitochondrial-CFP transfected cells treated with porphyrins revealed localization of TPPS1 in the peri-nuclear region, while TPPS4 localized in the mitochondria, inducing structural damage and swelling upon irradiation, as shown by transmission electron microscopy. TPPS4 fluorescence was detected in isolated mitochondria following irradiation. The photodamage induced a 38% reduction in mitochondrial activity, a 30% decrease in cellular ATP and a reduction in Na(+)/K(+)-ATPase activity. As a result, cytosolic concentrations of Na(+) and Ca(2+) increased, and the level of K(+) decreased. In contrast, the lipophilic TPPS1 did not affect...Continue Reading

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Citations

Feb 26, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rosa I Y Quiroz-SegovianoMiguel A García-Sánchez
Sep 26, 2008·Metal-based Drugs·Leanne B Josefsen, Ross W Boyle
Nov 12, 2016·The Breast : Official Journal of the European Society of Mastology·S M BanerjeeM R S Keshtgar
May 4, 2011·International Journal of Toxicology·Zhixiang ZhouRugang Zhong
Dec 31, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tet Htut SoeTakashi Ohtsuki
Jul 15, 2021·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Miryam Chiara MalacarneEnrico Caruso

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