Mitochondrial localization of vitamin D receptor in human platelets and differentiated megakaryocytes.

PloS One
Francesca SilvagnoGianpiero Pescarmona

Abstract

Like other steroid hormones, vitamin D elicits both transcriptional events and rapid non genomic effects. Vitamin D receptor (VDR) localization and mechanisms of VDR-triggered non genomic responses are still controversial. Although anticoagulant effects of vitamin D have been reported and VDR signalling has been characterized in monocytes and vascular cells, nothing is known about VDR expression and functions in human platelets, anucleated fragments of megakaryocytes which are known targets of other steroids. In this study we characterized the expression and cellular localization of VDR in human platelets and in a megakaryocyte lineage. Human platelets and their TPA-differentiated precursors expressed a classical 50 kDa VDR protein, which increased with megakaryocytes maturation. By biochemical fractionation studies we demonstrated the presence of the receptor in the soluble and mitochondrial compartment of human platelets, and the observation was confirmed by immunoelectron microscopy analysis. Similar localization was found in mature megakaryocytes, where besides its classical nuclear localization the receptor was evident as soluble and mitochondria resident protein. The results reported here suggest that megakaryocytopoiesis...Continue Reading

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Methods Mentioned

BETA
Flow Cytometry
Immunoprecipitation
electrophoresis
light-scattering
density gradient centrifugation
density gradient purification
electron microscopy

Software Mentioned

CellQuest
ImageJ

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